Dynamic label-free imaging of lipid nanodomains

Gabrielle De Wit; John S.H. Danial; Philipp Kukura; Mark I. Wallace

doi:10.1073/pnas.1508483112

Dynamic label-free imaging of lipid nanodomains

Gabrielle De Wit, John S.H. Danial, Philipp Kukura, Mark I. Wallace^*

^*Corresponding author for this work

Chemistry

University of Oxford

Research output: Contribution to journal › Article › peer-review

99 Citations (Scopus)

152 Downloads (Pure)

Abstract

Lipid rafts are submicron proteolipid domains thought to be responsible for membrane trafficking and signaling. Their small size and transient nature put an understanding of their dynamics beyond the reach of existing techniques, leading to much contention as to their exact role. Here, we exploit the differences in light scattering from lipid bilayer phases to achieve dynamic imaging of nanoscopic lipid domains without any labels. Using phase-separated droplet interface bilayers we resolve the diffusion of domains as small as nm in radius and observe nanodomain formation, destruction, and dynamic coalescence with a domain lifetime of 220 ± 60 ms. Domain dynamics on this timescale suggests an important role in modulating membrane protein function.

Original language	English
Pages (from-to)	12299-12303
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	112
Issue number	40
Early online date	23 Sept 2015
DOIs	https://doi.org/10.1073/pnas.1508483112
Publication status	Published - 6 Oct 2015

Keywords

Droplet interface bilayer
ISCAT
Label-free imaging
Light scattering
Lipid nanodomains

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AB - Lipid rafts are submicron proteolipid domains thought to be responsible for membrane trafficking and signaling. Their small size and transient nature put an understanding of their dynamics beyond the reach of existing techniques, leading to much contention as to their exact role. Here, we exploit the differences in light scattering from lipid bilayer phases to achieve dynamic imaging of nanoscopic lipid domains without any labels. Using phase-separated droplet interface bilayers we resolve the diffusion of domains as small as nm in radius and observe nanodomain formation, destruction, and dynamic coalescence with a domain lifetime of 220 ± 60 ms. Domain dynamics on this timescale suggests an important role in modulating membrane protein function.

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Dynamic label-free imaging of lipid nanodomains

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