Early raise of BDNF in hippocampus suggests induction of posttranscriptional mechanisms by antidepressants

TY - JOUR

T1 - Early raise of BDNF in hippocampus suggests induction of posttranscriptional mechanisms by antidepressants

AU - Musazzi, Laura

AU - Cattaneo, Annamaria

AU - Tardito, Daniela

AU - Barbon, Alessandro

AU - Gennarelli, Massimo

AU - Barlati, Sergio

AU - Racagni, Giorgio

AU - Popoli, Maurizio

PY - 2009/5

Y1 - 2009/5

N2 - The neurotrophin BDNF has been implicated in the regulation of neuroplasticity, gene expression, and synaptic function in the adult brain, as well as in the pathophysiology of neuropsychiatric disorders and the mechanism of action of antidepressants. Antidepressant treatments have been shown to increase the expression of BDNF mRNA, although the changes measured were found to be different depending on various factors. A few studies only have measured levels of BDNF protein after antidepressant treatments, and poor correlation was found between mRNA and protein changes. We studied the time course of expression of BDNF mRNA and protein during drug treatments, in order to elucidate the temporal profile of regulation of this effector and whether mRNA and protein levels correlate. Rat groups were treated for 1, 2 or 3 weeks with fluoxetine or reboxetine; in additional groups drug treatment was followed by a washout week (3+1). Total BDNF mRNA was measured by Real Time PCR, pro- and mature BDNF proteins were measured by Western blot.

AB - The neurotrophin BDNF has been implicated in the regulation of neuroplasticity, gene expression, and synaptic function in the adult brain, as well as in the pathophysiology of neuropsychiatric disorders and the mechanism of action of antidepressants. Antidepressant treatments have been shown to increase the expression of BDNF mRNA, although the changes measured were found to be different depending on various factors. A few studies only have measured levels of BDNF protein after antidepressant treatments, and poor correlation was found between mRNA and protein changes. We studied the time course of expression of BDNF mRNA and protein during drug treatments, in order to elucidate the temporal profile of regulation of this effector and whether mRNA and protein levels correlate. Rat groups were treated for 1, 2 or 3 weeks with fluoxetine or reboxetine; in additional groups drug treatment was followed by a washout week (3+1). Total BDNF mRNA was measured by Real Time PCR, pro- and mature BDNF proteins were measured by Western blot.

U2 - 10.1186/1471-2202-10-48

DO - 10.1186/1471-2202-10-48

M3 - Article

C2 - 19439074

VL - 10

JO - BMC NEUROSCIENCE

JF - BMC NEUROSCIENCE

M1 - 48

ER -