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Effect of PGC1-beta ablation on myonuclear organisation

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Effect of PGC1-beta ablation on myonuclear organisation. / Beedour, Ryan; Ross, Jacob A.; Levy, Yotam; Ochala, Julien.

In: Journal of Muscle Research and Cell Motility, Vol. 40, No. 3-4, 01.12.2019, p. 335-341.

Research output: Contribution to journalArticle

Harvard

Beedour, R, Ross, JA, Levy, Y & Ochala, J 2019, 'Effect of PGC1-beta ablation on myonuclear organisation', Journal of Muscle Research and Cell Motility, vol. 40, no. 3-4, pp. 335-341. https://doi.org/10.1007/s10974-019-09549-3

APA

Beedour, R., Ross, J. A., Levy, Y., & Ochala, J. (2019). Effect of PGC1-beta ablation on myonuclear organisation. Journal of Muscle Research and Cell Motility, 40(3-4), 335-341. https://doi.org/10.1007/s10974-019-09549-3

Vancouver

Beedour R, Ross JA, Levy Y, Ochala J. Effect of PGC1-beta ablation on myonuclear organisation. Journal of Muscle Research and Cell Motility. 2019 Dec 1;40(3-4):335-341. https://doi.org/10.1007/s10974-019-09549-3

Author

Beedour, Ryan ; Ross, Jacob A. ; Levy, Yotam ; Ochala, Julien. / Effect of PGC1-beta ablation on myonuclear organisation. In: Journal of Muscle Research and Cell Motility. 2019 ; Vol. 40, No. 3-4. pp. 335-341.

Bibtex Download

@article{0fce8bfaf1924aacb1c88325039cab9d,
title = "Effect of PGC1-beta ablation on myonuclear organisation",
abstract = "Skeletal muscle fibres are large, elongated multinucleated cells. Each nucleus within a myofibre is responsible for generating gene products for a finite volume of cytoplasm—the myonuclear domain (MND). Variation in MND sizes during atrophy, hypertrophy and disease states, are common. The factors that contribute to definitive MND sizes are not yet fully understood. Previous work has shown that peroxisome proliferator-activated receptor gamma coactivator 1α (PGC1-α) modulates MND volume, presumably to support increased biogenesis of mitochondria. The transcriptional co-regulator peroxisome proliferator-activated receptor gamma coactivator 1β (PGC1-β) is a homologue of PGC1-α with overlapping functions. To investigate the role of this protein in MND size regulation, we studied a mouse skeletal muscle specific knockout (cKO). Myofibres were isolated from the fast twitch extensor digitorum longus (EDL) muscle, membrane-permeabilised and analysed in 3 dimensions using confocal microscopy. PGC1-β ablation resulted in no significant difference in MND size between cKO and wild type (WT) mice, however, subtle differences in nuclear morphology were observed. To determine whether these nuclear shape changes were associated with alterations in global transcriptional activity, acetyl histone H3 immunostaining was carried out. We found there was no significant difference in nuclear fluorescence intensity between the two genotypes. Overall, the results suggest that PGC-1α and PGC-1β play different roles in regulating nuclear organisation in skeletal muscle; however, further work is required to pinpoint their exact functions.",
keywords = "Myofibre, Myonuclear domain, Nuclear shape, PGC1-beta",
author = "Ryan Beedour and Ross, {Jacob A.} and Yotam Levy and Julien Ochala",
year = "2019",
month = "12",
day = "1",
doi = "10.1007/s10974-019-09549-3",
language = "English",
volume = "40",
pages = "335--341",
journal = "Journal of Muscle Research and Cell Motility",
issn = "0142-4319",
publisher = "Springer Netherlands",
number = "3-4",

}

RIS (suitable for import to EndNote) Download

TY - JOUR

T1 - Effect of PGC1-beta ablation on myonuclear organisation

AU - Beedour, Ryan

AU - Ross, Jacob A.

AU - Levy, Yotam

AU - Ochala, Julien

PY - 2019/12/1

Y1 - 2019/12/1

N2 - Skeletal muscle fibres are large, elongated multinucleated cells. Each nucleus within a myofibre is responsible for generating gene products for a finite volume of cytoplasm—the myonuclear domain (MND). Variation in MND sizes during atrophy, hypertrophy and disease states, are common. The factors that contribute to definitive MND sizes are not yet fully understood. Previous work has shown that peroxisome proliferator-activated receptor gamma coactivator 1α (PGC1-α) modulates MND volume, presumably to support increased biogenesis of mitochondria. The transcriptional co-regulator peroxisome proliferator-activated receptor gamma coactivator 1β (PGC1-β) is a homologue of PGC1-α with overlapping functions. To investigate the role of this protein in MND size regulation, we studied a mouse skeletal muscle specific knockout (cKO). Myofibres were isolated from the fast twitch extensor digitorum longus (EDL) muscle, membrane-permeabilised and analysed in 3 dimensions using confocal microscopy. PGC1-β ablation resulted in no significant difference in MND size between cKO and wild type (WT) mice, however, subtle differences in nuclear morphology were observed. To determine whether these nuclear shape changes were associated with alterations in global transcriptional activity, acetyl histone H3 immunostaining was carried out. We found there was no significant difference in nuclear fluorescence intensity between the two genotypes. Overall, the results suggest that PGC-1α and PGC-1β play different roles in regulating nuclear organisation in skeletal muscle; however, further work is required to pinpoint their exact functions.

AB - Skeletal muscle fibres are large, elongated multinucleated cells. Each nucleus within a myofibre is responsible for generating gene products for a finite volume of cytoplasm—the myonuclear domain (MND). Variation in MND sizes during atrophy, hypertrophy and disease states, are common. The factors that contribute to definitive MND sizes are not yet fully understood. Previous work has shown that peroxisome proliferator-activated receptor gamma coactivator 1α (PGC1-α) modulates MND volume, presumably to support increased biogenesis of mitochondria. The transcriptional co-regulator peroxisome proliferator-activated receptor gamma coactivator 1β (PGC1-β) is a homologue of PGC1-α with overlapping functions. To investigate the role of this protein in MND size regulation, we studied a mouse skeletal muscle specific knockout (cKO). Myofibres were isolated from the fast twitch extensor digitorum longus (EDL) muscle, membrane-permeabilised and analysed in 3 dimensions using confocal microscopy. PGC1-β ablation resulted in no significant difference in MND size between cKO and wild type (WT) mice, however, subtle differences in nuclear morphology were observed. To determine whether these nuclear shape changes were associated with alterations in global transcriptional activity, acetyl histone H3 immunostaining was carried out. We found there was no significant difference in nuclear fluorescence intensity between the two genotypes. Overall, the results suggest that PGC-1α and PGC-1β play different roles in regulating nuclear organisation in skeletal muscle; however, further work is required to pinpoint their exact functions.

KW - Myofibre

KW - Myonuclear domain

KW - Nuclear shape

KW - PGC1-beta

UR - http://www.scopus.com/inward/record.url?scp=85071766689&partnerID=8YFLogxK

U2 - 10.1007/s10974-019-09549-3

DO - 10.1007/s10974-019-09549-3

M3 - Article

C2 - 31485877

AN - SCOPUS:85071766689

VL - 40

SP - 335

EP - 341

JO - Journal of Muscle Research and Cell Motility

JF - Journal of Muscle Research and Cell Motility

SN - 0142-4319

IS - 3-4

ER -

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