TY - JOUR
T1 - Effect of zinc depletion/repletion on intestinal iron absorption and iron status in rats
AU - Kondaiah, Palsa
AU - Palika, Ravindranadh
AU - Mashurabad, Purnachandra
AU - Singh Yaduvanshi, Puneeta
AU - Sharp, Paul
AU - Pullakhandam, Raghu
N1 - Funding Information:
This study was supported in part by intramural grants of NIN-Indian Council of Medical Research (12/BS03) and Department of Biotechnology [BT/PR12128/PEN/20/960/2014], Govt of India. K.P. (SRF) and PSY (JRF) are supported by University Grants Commission, Govt of India.
Publisher Copyright:
© 2021
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021/11
Y1 - 2021/11
N2 - Iron and zinc deficiencies likely coexist in general population. We have previously demonstrated that zinc treatment induces while zinc deficiency inhibits iron absorption in intestinal cell culture models, but this needs to be tested in vivo. In the present study we assessed intestinal iron absorption, iron status (haemoglobin), red blood cell number, plasma ferritin, transferrin receptor, hepcidin) and tissue iron levels in zinc depleted, replete and pair fed control rats. Zinc depletion led to reduction in body weight, tissue zinc levels, intestinal iron absorption, protein and mRNA expression of iron transporters, the divalent metal ion transporter-1, hephaestin and ferroportin, but elevated the intestinal and liver tissue iron levels compared with the pair fed control rats. Zinc repletion led to a significant weight gain compared to zinc deficient rats and normalized the iron absorption, iron transporter expression, tissue iron levels to that of pair fed control rats. Surprisingly, haemoglobin levels and red blood cell number reduced significantly in zinc repleted rats, which could be due to rapid weight gain. Together, these results indicate that whole body zinc status has profound influence on growth, intestinal absorption and systemic utilization of iron, mediated via modulation of iron transporter expression.
AB - Iron and zinc deficiencies likely coexist in general population. We have previously demonstrated that zinc treatment induces while zinc deficiency inhibits iron absorption in intestinal cell culture models, but this needs to be tested in vivo. In the present study we assessed intestinal iron absorption, iron status (haemoglobin), red blood cell number, plasma ferritin, transferrin receptor, hepcidin) and tissue iron levels in zinc depleted, replete and pair fed control rats. Zinc depletion led to reduction in body weight, tissue zinc levels, intestinal iron absorption, protein and mRNA expression of iron transporters, the divalent metal ion transporter-1, hephaestin and ferroportin, but elevated the intestinal and liver tissue iron levels compared with the pair fed control rats. Zinc repletion led to a significant weight gain compared to zinc deficient rats and normalized the iron absorption, iron transporter expression, tissue iron levels to that of pair fed control rats. Surprisingly, haemoglobin levels and red blood cell number reduced significantly in zinc repleted rats, which could be due to rapid weight gain. Together, these results indicate that whole body zinc status has profound influence on growth, intestinal absorption and systemic utilization of iron, mediated via modulation of iron transporter expression.
KW - Depletion/repletion
KW - Interactions
KW - Intestinal iron absorption
KW - Iron
KW - Iron transporters
KW - Zinc
UR - http://www.scopus.com/inward/record.url?scp=85108989854&partnerID=8YFLogxK
U2 - 10.1016/j.jnutbio.2021.108800
DO - 10.1016/j.jnutbio.2021.108800
M3 - Article
C2 - 34118433
AN - SCOPUS:85108989854
SN - 0955-2863
VL - 97
JO - JOURNAL OF NUTRITIONAL BIOCHEMISTRY
JF - JOURNAL OF NUTRITIONAL BIOCHEMISTRY
M1 - 108800
ER -