Effective experimental design: Enzyme kinetics in the bioinformatics era

TY - JOUR

T1 - Effective experimental design

T2 - Enzyme kinetics in the bioinformatics era

AU - Murphy, Emma F.

AU - Gilmour, Steven G.

AU - Crabbe, M. James C

PY - 2002/10/15

Y1 - 2002/10/15

N2 - Acquiring details about the kinetic parameters of enzymes is crucial to both drug development and clinical diagnosis. The correct design of an experiment is crucial to collecting data suitable for analysis, modelling and deriving the correct information. As classical design methods are not targeted to the more complex kinetics now frequently studied, further work is required to estimate parameters of such models with low variance. This review examines the different options available to produce major gains in information, productivity and the accuracy of each experiment.

AB - Acquiring details about the kinetic parameters of enzymes is crucial to both drug development and clinical diagnosis. The correct design of an experiment is crucial to collecting data suitable for analysis, modelling and deriving the correct information. As classical design methods are not targeted to the more complex kinetics now frequently studied, further work is required to estimate parameters of such models with low variance. This review examines the different options available to produce major gains in information, productivity and the accuracy of each experiment.

UR - http://www.scopus.com/inward/record.url?scp=0037107884&partnerID=8YFLogxK

U2 - 10.1016/S1359-6446(02)02384-X

DO - 10.1016/S1359-6446(02)02384-X

M3 - Article

C2 - 12546904

AN - SCOPUS:0037107884

SN - 1359-6446

VL - 7

JO - DRUG DISCOVERY TODAY

JF - DRUG DISCOVERY TODAY

IS - 20

ER -