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Effects of beta-adrenoceptor stimulation on endothelial nitric-oxide synthase phosphorylation of human umbilical vein endothelial cells

Research output: Contribution to journalArticle

K Yao, B Xu, Y P Liu, A Ferro

Original languageEnglish
Pages (from-to)219 - 224
Number of pages6
Issue number3
Publication statusPublished - 2003

King's Authors


AIM: To investigate the effects of isoprenaline on the phosphorylation level of endothelial nitric oxide synthase (eNOS) of human umbilical vein endothelial cells (HUVEC) and to elucidate the regulation mechanisms of beta-adrenoceptor (beta-AR) on eNOS activity. METHODS: HUVEC were exposed to isoprenaline (1 mumol/L) for 30 min, in the absence or presence of selective beta(1) or beta(2) -adrenoceptor antagonists. eNOS was then isolated by immunoprecipitation using a specific eNOS antibody. eNOS activity was determined by the conversion of L-[H-3] arginine to L- [H-3]citrulline. The levels of eNOS expression and eNOS phosphorylation were measured by Western blotting. RESULTS: Treatment of HUVEC with isoprenaline (1 mumol/L) for 30 min caused no measureable change in eNOS expression. However, eNOS activity and serine phosphorylation level were significantly increased (P

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