Effects of macrophage colony-stimulating factor and phorbol myristate acetate on 2-D-deoxyglucose transport and superoxide production in rat peritoneal macrophages

TY - JOUR

T1 - Effects of macrophage colony-stimulating factor and phorbol myristate acetate on 2-D-deoxyglucose transport and superoxide production in rat peritoneal macrophages

AU - Rist, R. J.

AU - Jones, G. E.

AU - Naftalin, R. J.

PY - 1991/1/1

Y1 - 1991/1/1

N2 - 2-D-Deoxyglucose (2-dGlc) uptake and accumulation into rat peritoneal macrophages was increased by colony-stimulating factor (mCSF) by stimulating the coupling between endofacial hexokinase activity and the sugar transporter. The evidence for this is as follows: (1) mCSF significantly decreased the K(m) for zero-trans uptake (P < 0.05), without altering V(max); (2) the accumulation of free 2-dGlc was increased by mCSF (P < 0.05); (3) mCSF retarded the rate of exit of accumulated free 2-dGlc. The mCSF-dependent increase in 2-dGlc uptake by macrophages was enhanced by preincubation of the cells in mCSF-free solution. The activity of the hexose monophosphate shunt (HMPS) measured by the differential uptake of 2-d[1-3H]Glc and 2-d[2,6-3H]Glc was not stimulated by mCSF. Also, in quiescent cells, superoxide production, as determined by cytochrome c reduction, was unaffected by mCSF. Phorbol myristate acetate (PMA; 40 nM) stimulated both the HMPS activity and superoxide production. Both these effects were dependent on the uptake of external sugar (2-dGlc). Incubation of the macrophages with mCSF enhanced the sugar transport and PMA-dependent stimulation of HMPS activity and superoxide production, indicating a role for mCSF in the 'priming' of macrophage functions. Both HMPS activity and superoxide production are entirely dependent on uptake of s sugar, since the potent sugar-transport inhibitor cytochalasin B competitively inhibited 2-dGlc uptake, HMPS activity and superoxide generation in PMA-activated cells (K(i) ~ 0.3 μM for all three processes). Over a wide range of 2-dGlc concentrations, 4 mol of superoxide were generated/mol of 2-dGlc metabolized in the HMPS pathway, indicating coupling between these processes. The K(m) of 2-d[2,6-3H]Glc uptake in PMA-treated cells was 0.45 ± 0.07 mM, and V(max) was 1.32 ± 0.05 μmol · min-1. ml of cell water-1. It is evident that there is a large degree of slippage between HMPS activity and membrane-associated hexokinase activity, since the K(m) for HMPS activity was 0.06 ± 0.02 mM and the V(max) was 0.10 ± 0.03 μmol · min-1 · ml of cell water-1.

AB - 2-D-Deoxyglucose (2-dGlc) uptake and accumulation into rat peritoneal macrophages was increased by colony-stimulating factor (mCSF) by stimulating the coupling between endofacial hexokinase activity and the sugar transporter. The evidence for this is as follows: (1) mCSF significantly decreased the K(m) for zero-trans uptake (P < 0.05), without altering V(max); (2) the accumulation of free 2-dGlc was increased by mCSF (P < 0.05); (3) mCSF retarded the rate of exit of accumulated free 2-dGlc. The mCSF-dependent increase in 2-dGlc uptake by macrophages was enhanced by preincubation of the cells in mCSF-free solution. The activity of the hexose monophosphate shunt (HMPS) measured by the differential uptake of 2-d[1-3H]Glc and 2-d[2,6-3H]Glc was not stimulated by mCSF. Also, in quiescent cells, superoxide production, as determined by cytochrome c reduction, was unaffected by mCSF. Phorbol myristate acetate (PMA; 40 nM) stimulated both the HMPS activity and superoxide production. Both these effects were dependent on the uptake of external sugar (2-dGlc). Incubation of the macrophages with mCSF enhanced the sugar transport and PMA-dependent stimulation of HMPS activity and superoxide production, indicating a role for mCSF in the 'priming' of macrophage functions. Both HMPS activity and superoxide production are entirely dependent on uptake of s sugar, since the potent sugar-transport inhibitor cytochalasin B competitively inhibited 2-dGlc uptake, HMPS activity and superoxide generation in PMA-activated cells (K(i) ~ 0.3 μM for all three processes). Over a wide range of 2-dGlc concentrations, 4 mol of superoxide were generated/mol of 2-dGlc metabolized in the HMPS pathway, indicating coupling between these processes. The K(m) of 2-d[2,6-3H]Glc uptake in PMA-treated cells was 0.45 ± 0.07 mM, and V(max) was 1.32 ± 0.05 μmol · min-1. ml of cell water-1. It is evident that there is a large degree of slippage between HMPS activity and membrane-associated hexokinase activity, since the K(m) for HMPS activity was 0.06 ± 0.02 mM and the V(max) was 0.10 ± 0.03 μmol · min-1 · ml of cell water-1.

UR - http://www.scopus.com/inward/record.url?scp=0025880471&partnerID=8YFLogxK

U2 - 10.1042/bj2780119

DO - 10.1042/bj2780119

M3 - Article

C2 - 1652936

AN - SCOPUS:0025880471

SN - 0264-6021

VL - 278

SP - 119

EP - 128

JO - Biochemical Journal

JF - Biochemical Journal

IS - 1

ER -