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Effects of maternal obesity on Wharton's Jelly mesenchymal stromal cells

Research output: Contribution to journalArticlepeer-review

Original languageEnglish
Article number17595
JournalScientific Reports
Volume7
Issue number1
Early online date14 Dec 2017
DOIs
Accepted/In press5 Dec 2017
E-pub ahead of print14 Dec 2017
PublishedDec 2017

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  • Effects of maternal obesity on_BADRAIQ_Publishedonline14December2017_GOLD VoR (CC BY)

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    Uploaded date:04 Jan 2018

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    Licence:CC BY

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    © The Author(s) 2017

King's Authors

Abstract

We investigated whether maternal metabolic environment affects mesenchymal stromal/stem cells (MSCs) from umbilical cord's Wharton's Jelly (WJ) on a molecular level, and potentially render them unsuitable for clinical use in multiple recipients. In this pilot study on umbilical cords post partum from healthy non-obese (BMI = 19-25; n = 7) and obese (BMI ≥ 30; n = 7) donors undergoing elective Cesarean section, we found that WJ MSC from obese donors showed slower population doubling and a stronger immunosuppressive activity. Genome-wide DNA methylation of triple positive (CD73+CD90+CD105+) WJ MSCs found 67 genes with at least one CpG site where the methylation difference was ≥0.2 in four or more obese donors. Only one gene, PNPLA7, demonstrated significant difference on methylome, transcriptome and protein level. Although the number of analysed donors is limited, our data suggest that the altered metabolic environment related to excessive body weight might bear consequences on the WJ MSCs.

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