Engineering Anisotropic Muscle Tissue using Acoustic Cell Patterning

James P. Armstrong; Jennifer L. Puetzer; Andrea Serio; Anne G. Guex; Mikaella  Kapnisi; Alexander Breant; Yifan Zong; Stacey Skaalure; Oisin King; null Murty; null Meinert; null Franklin; null Bassindale; null Nichols; Cesare Mn Terracciano; null Hutmacher; null Drinkwater; null Klein; Adam W. Perriman; Molly Stevens

doi:10.1002/adma.201802649

Engineering Anisotropic Muscle Tissue using Acoustic Cell Patterning

James P. Armstrong, Jennifer L. Puetzer, Andrea Serio, Anne G. Guex, Mikaella Kapnisi, Alexander Breant, Yifan Zong, Stacey Skaalure, Oisin King, Murty, Meinert, Franklin, Bassindale, Nichols, Cesare Mn Terracciano, Hutmacher, Drinkwater, Klein, Adam W. Perriman, Molly Stevens

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Abstract

Tissue engineering has offered unique opportunities for disease modeling and regenerative medicine; however, the success of these strategies is dependent on faithful reproduction of native cellular organization. Here, it is reported that ultrasound standing waves can be used to organize myoblast populations in material systems for the engineering of aligned muscle tissue constructs. Patterned muscle engineered using type I collagen hydrogels exhibits significant anisotropy in tensile strength, and under mechanical constraint, produced microscale alignment on a cell and fiber level. Moreover, acoustic patterning of myoblasts in gelatin methacryloyl hydrogels significantly enhances myofibrillogenesis and promotes the formation of muscle fibers containing aligned bundles of myotubes, with a width of 120–150 µm and a spacing of 180–220 µm. The ability to remotely pattern fibers of aligned myotubes without any material cues or complex fabrication procedures represents a significant advance in the field of muscle tissue engineering. In general, these results are the first instance of engineered cell fibers formed from the differentiation of acoustically patterned cells. It is anticipated that this versatile methodology can be applied to many complex tissue morphologies, with broader relevance for spatially organized cell cultures, organoid development, and bioelectronics.

Original language	English
Article number	1802649
Number of pages	7
Journal	ADVANCED MATERIALS
Volume	30
Issue number	43
Early online date	12 Sept 2018
DOIs	https://doi.org/10.1002/adma.201802649
Publication status	Published - 25 Oct 2018

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Engineering Anisotropic Muscle Tissue_ARMSTRONG_Published Online 25 October 2018_ GOLD VoR (CC BY)Final published version, 2 MBLicence: CC BY

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Armstrong, J. P., Puetzer, J. L., Serio, A., Guex, A. G., Kapnisi, M., Breant, A., Zong, Y., Skaalure, S., King, O., Murty, Meinert, Franklin, Bassindale, Nichols, Terracciano, C. M., Hutmacher, Drinkwater, Klein, Perriman, A. W., & Stevens, M. (2018). Engineering Anisotropic Muscle Tissue using Acoustic Cell Patterning. ADVANCED MATERIALS, 30(43), Article 1802649. https://doi.org/10.1002/adma.201802649

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title = "Engineering Anisotropic Muscle Tissue using Acoustic Cell Patterning",

abstract = "Tissue engineering has offered unique opportunities for disease modeling and regenerative medicine; however, the success of these strategies is dependent on faithful reproduction of native cellular organization. Here, it is reported that ultrasound standing waves can be used to organize myoblast populations in material systems for the engineering of aligned muscle tissue constructs. Patterned muscle engineered using type I collagen hydrogels exhibits significant anisotropy in tensile strength, and under mechanical constraint, produced microscale alignment on a cell and fiber level. Moreover, acoustic patterning of myoblasts in gelatin methacryloyl hydrogels significantly enhances myofibrillogenesis and promotes the formation of muscle fibers containing aligned bundles of myotubes, with a width of 120–150 µm and a spacing of 180–220 µm. The ability to remotely pattern fibers of aligned myotubes without any material cues or complex fabrication procedures represents a significant advance in the field of muscle tissue engineering. In general, these results are the first instance of engineered cell fibers formed from the differentiation of acoustically patterned cells. It is anticipated that this versatile methodology can be applied to many complex tissue morphologies, with broader relevance for spatially organized cell cultures, organoid development, and bioelectronics.",

author = "Armstrong, {James P.} and Puetzer, {Jennifer L.} and Andrea Serio and Guex, {Anne G.} and Mikaella Kapnisi and Alexander Breant and Yifan Zong and Stacey Skaalure and Oisin King and Murty and Meinert and Franklin and Bassindale and Nichols and Terracciano, {Cesare Mn} and Hutmacher and Drinkwater and Klein and Perriman, {Adam W.} and Molly Stevens",

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doi = "10.1002/adma.201802649",

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Armstrong, JP, Puetzer, JL, Serio, A, Guex, AG, Kapnisi, M, Breant, A, Zong, Y, Skaalure, S, King, O, Murty, Meinert, Franklin, Bassindale, Nichols, Terracciano, CM, Hutmacher, Drinkwater, Klein, Perriman, AW & Stevens, M 2018, 'Engineering Anisotropic Muscle Tissue using Acoustic Cell Patterning', ADVANCED MATERIALS, vol. 30, no. 43, 1802649. https://doi.org/10.1002/adma.201802649

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T1 - Engineering Anisotropic Muscle Tissue using Acoustic Cell Patterning

AU - Armstrong, James P.

AU - Puetzer, Jennifer L.

AU - Serio, Andrea

AU - Guex, Anne G.

AU - Kapnisi, Mikaella

AU - Breant, Alexander

AU - Zong, Yifan

AU - Skaalure, Stacey

AU - King, Oisin

AU - Murty, null

AU - Meinert, null

AU - Franklin, null

AU - Bassindale, null

AU - Nichols, null

AU - Terracciano, Cesare Mn

AU - Hutmacher, null

AU - Drinkwater, null

AU - Klein, null

AU - Perriman, Adam W.

AU - Stevens, Molly

PY - 2018/10/25

Y1 - 2018/10/25

N2 - Tissue engineering has offered unique opportunities for disease modeling and regenerative medicine; however, the success of these strategies is dependent on faithful reproduction of native cellular organization. Here, it is reported that ultrasound standing waves can be used to organize myoblast populations in material systems for the engineering of aligned muscle tissue constructs. Patterned muscle engineered using type I collagen hydrogels exhibits significant anisotropy in tensile strength, and under mechanical constraint, produced microscale alignment on a cell and fiber level. Moreover, acoustic patterning of myoblasts in gelatin methacryloyl hydrogels significantly enhances myofibrillogenesis and promotes the formation of muscle fibers containing aligned bundles of myotubes, with a width of 120–150 µm and a spacing of 180–220 µm. The ability to remotely pattern fibers of aligned myotubes without any material cues or complex fabrication procedures represents a significant advance in the field of muscle tissue engineering. In general, these results are the first instance of engineered cell fibers formed from the differentiation of acoustically patterned cells. It is anticipated that this versatile methodology can be applied to many complex tissue morphologies, with broader relevance for spatially organized cell cultures, organoid development, and bioelectronics.

AB - Tissue engineering has offered unique opportunities for disease modeling and regenerative medicine; however, the success of these strategies is dependent on faithful reproduction of native cellular organization. Here, it is reported that ultrasound standing waves can be used to organize myoblast populations in material systems for the engineering of aligned muscle tissue constructs. Patterned muscle engineered using type I collagen hydrogels exhibits significant anisotropy in tensile strength, and under mechanical constraint, produced microscale alignment on a cell and fiber level. Moreover, acoustic patterning of myoblasts in gelatin methacryloyl hydrogels significantly enhances myofibrillogenesis and promotes the formation of muscle fibers containing aligned bundles of myotubes, with a width of 120–150 µm and a spacing of 180–220 µm. The ability to remotely pattern fibers of aligned myotubes without any material cues or complex fabrication procedures represents a significant advance in the field of muscle tissue engineering. In general, these results are the first instance of engineered cell fibers formed from the differentiation of acoustically patterned cells. It is anticipated that this versatile methodology can be applied to many complex tissue morphologies, with broader relevance for spatially organized cell cultures, organoid development, and bioelectronics.

U2 - 10.1002/adma.201802649

DO - 10.1002/adma.201802649

M3 - Article

SN - 0935-9648

VL - 30

JO - ADVANCED MATERIALS

JF - ADVANCED MATERIALS

IS - 43

M1 - 1802649

ER -

Engineering Anisotropic Muscle Tissue using Acoustic Cell Patterning

Abstract

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