TY - JOUR
T1 - Engineering Controllable Pulse Generation for Molecular Communication via Genetic Circuits
AU - Bi, Dadi
AU - Deng, Yansha
N1 - Funding Information:
This work was supported by the Engineering and Physical Sciences Research Council (EPSRC), U.K., under Grant EP/T000937/1.
Publisher Copyright:
© 2015 IEEE.
PY - 2023/2/17
Y1 - 2023/2/17
N2 - Synthetic biology provides the engineering community with a tool to build biological entities that are capable of carrying out desired signal processing functionalities. The design and study of genetic circuits that exhibit natural behavior can be helpful for an improved understanding of the principles and kinetics behind the gene expression behavior, as well as for engineering cellular systems for synthetic biology. In this paper, we propose a synthetic biology system capable of generating a pulse-shaped signal, which is a prevalent behavior in natural environment. In particular, our proposed pulse generation system divides the pulse generation functions into different cells which are connected by intercellular signaling molecules. More specifically, the system is consisted of three engineered cells with different digital logic functionalities, and the pulse generation exploits the alteration in logic state. To quantitatively describe the generated pulse, we not only analyze the individual behavior of each cell using Shea-Ackers formalism but also derive the intercellular signaling channel. Simulation results demonstrate that the pulse-shaped signal can be produced in a controllable manner by arranging cells in different spatial configurations.
AB - Synthetic biology provides the engineering community with a tool to build biological entities that are capable of carrying out desired signal processing functionalities. The design and study of genetic circuits that exhibit natural behavior can be helpful for an improved understanding of the principles and kinetics behind the gene expression behavior, as well as for engineering cellular systems for synthetic biology. In this paper, we propose a synthetic biology system capable of generating a pulse-shaped signal, which is a prevalent behavior in natural environment. In particular, our proposed pulse generation system divides the pulse generation functions into different cells which are connected by intercellular signaling molecules. More specifically, the system is consisted of three engineered cells with different digital logic functionalities, and the pulse generation exploits the alteration in logic state. To quantitatively describe the generated pulse, we not only analyze the individual behavior of each cell using Shea-Ackers formalism but also derive the intercellular signaling channel. Simulation results demonstrate that the pulse-shaped signal can be produced in a controllable manner by arranging cells in different spatial configurations.
KW - Digital logic circuits
KW - gene expression
KW - genetic circuits
KW - molecular communication
KW - pulse generation
KW - synthetic biology
UR - http://www.scopus.com/inward/record.url?scp=85149860660&partnerID=8YFLogxK
U2 - 10.1109/TMBMC.2023.3252942
DO - 10.1109/TMBMC.2023.3252942
M3 - Article
AN - SCOPUS:85149860660
SN - 2332-7804
VL - 9
SP - 79
EP - 93
JO - IEEE Transactions on Molecular, Biological, and Multi-Scale Communications
JF - IEEE Transactions on Molecular, Biological, and Multi-Scale Communications
IS - 1
ER -