Enhanced antibody responses to Plasmodium falciparum Pfs28 induced in mice by conjugation to ExoProtein A of Pseudomonas aeruginosa with an improved procedure

F Qian; JA Aebig; K Reiter; E Barnafo; Y Zhang; RL Jr Shimp; KM Rausch; DS Jones; D Zhu; L Lambert; GE Mullen; DL Narum; LH Miller; Y Wu

doi:10.1016/j.micinf.2008.12.009

Enhanced antibody responses to Plasmodium falciparum Pfs28 induced in mice by conjugation to ExoProtein A of Pseudomonas aeruginosa with an improved procedure

F Qian, JA Aebig, K Reiter, E Barnafo, Y Zhang, RL Jr Shimp, KM Rausch, DS Jones, D Zhu, L Lambert, GE Mullen, DL Narum, LH Miller, Y Wu

Imaging Chemistry & Biology

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Abstract

In this paper we report our efforts to enhance the immunogenicity of Pfs28, a transmission blocking vaccine candidate of Plasmodium falciparum, using a strategy of chemical conjugation. With an improved procedure, Pfs28 was covalently coupled to the mutant and non-toxic ExoProtein A of Pseudomonas aeruginosa by the reaction between thiolated antigen and maleimide modified carrier protein. The optimized process resulted in a higher antigen-carrier conjugation ratio, and the conjugation product could be purified using single-step size-exclusion chromatography. A significant increase in immunogenicity measured by ELISA was observed in mice immunized with conjugated Pfs28 as compared to unconjugated Pfs28. Published by Elsevier Masson SAS.

Original language	English
Pages (from-to)	408-412
Number of pages	5
Journal	MICROBES AND INFECTION
Volume	11
Issue number	3
DOIs	https://doi.org/10.1016/j.micinf.2008.12.009
Publication status	Published - Mar 2009

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10.1016/j.micinf.2008.12.009

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Qian, F., Aebig, JA., Reiter, K., Barnafo, E., Zhang, Y., Shimp, RL. J., Rausch, KM., Jones, DS., Zhu, D., Lambert, L., Mullen, GE., Narum, DL., Miller, LH., & Wu, Y. (2009). Enhanced antibody responses to Plasmodium falciparum Pfs28 induced in mice by conjugation to ExoProtein A of Pseudomonas aeruginosa with an improved procedure. MICROBES AND INFECTION, 11(3), 408-412. https://doi.org/10.1016/j.micinf.2008.12.009

@article{e4214a46ae444826ad5b6f578393cd7b,

title = "Enhanced antibody responses to Plasmodium falciparum Pfs28 induced in mice by conjugation to ExoProtein A of Pseudomonas aeruginosa with an improved procedure",

abstract = "In this paper we report our efforts to enhance the immunogenicity of Pfs28, a transmission blocking vaccine candidate of Plasmodium falciparum, using a strategy of chemical conjugation. With an improved procedure, Pfs28 was covalently coupled to the mutant and non-toxic ExoProtein A of Pseudomonas aeruginosa by the reaction between thiolated antigen and maleimide modified carrier protein. The optimized process resulted in a higher antigen-carrier conjugation ratio, and the conjugation product could be purified using single-step size-exclusion chromatography. A significant increase in immunogenicity measured by ELISA was observed in mice immunized with conjugated Pfs28 as compared to unconjugated Pfs28. Published by Elsevier Masson SAS.",

author = "F Qian and JA Aebig and K Reiter and E Barnafo and Y Zhang and Shimp, {RL Jr} and KM Rausch and DS Jones and D Zhu and L Lambert and GE Mullen and DL Narum and LH Miller and Y Wu",

year = "2009",

month = mar,

doi = "10.1016/j.micinf.2008.12.009",

language = "English",

volume = "11",

pages = "408--412",

journal = "MICROBES AND INFECTION",

publisher = "Elsevier Masson SAS",

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Qian, F, Aebig, JA, Reiter, K, Barnafo, E, Zhang, Y, Shimp, RLJ, Rausch, KM, Jones, DS, Zhu, D, Lambert, L, Mullen, GE, Narum, DL, Miller, LH & Wu, Y 2009, 'Enhanced antibody responses to Plasmodium falciparum Pfs28 induced in mice by conjugation to ExoProtein A of Pseudomonas aeruginosa with an improved procedure', MICROBES AND INFECTION, vol. 11, no. 3, pp. 408-412. https://doi.org/10.1016/j.micinf.2008.12.009

TY - JOUR

T1 - Enhanced antibody responses to Plasmodium falciparum Pfs28 induced in mice by conjugation to ExoProtein A of Pseudomonas aeruginosa with an improved procedure

AU - Qian, F

AU - Aebig, JA

AU - Reiter, K

AU - Barnafo, E

AU - Zhang, Y

AU - Shimp, RL Jr

AU - Rausch, KM

AU - Jones, DS

AU - Zhu, D

AU - Lambert, L

AU - Mullen, GE

AU - Narum, DL

AU - Miller, LH

AU - Wu, Y

PY - 2009/3

Y1 - 2009/3

N2 - In this paper we report our efforts to enhance the immunogenicity of Pfs28, a transmission blocking vaccine candidate of Plasmodium falciparum, using a strategy of chemical conjugation. With an improved procedure, Pfs28 was covalently coupled to the mutant and non-toxic ExoProtein A of Pseudomonas aeruginosa by the reaction between thiolated antigen and maleimide modified carrier protein. The optimized process resulted in a higher antigen-carrier conjugation ratio, and the conjugation product could be purified using single-step size-exclusion chromatography. A significant increase in immunogenicity measured by ELISA was observed in mice immunized with conjugated Pfs28 as compared to unconjugated Pfs28. Published by Elsevier Masson SAS.

AB - In this paper we report our efforts to enhance the immunogenicity of Pfs28, a transmission blocking vaccine candidate of Plasmodium falciparum, using a strategy of chemical conjugation. With an improved procedure, Pfs28 was covalently coupled to the mutant and non-toxic ExoProtein A of Pseudomonas aeruginosa by the reaction between thiolated antigen and maleimide modified carrier protein. The optimized process resulted in a higher antigen-carrier conjugation ratio, and the conjugation product could be purified using single-step size-exclusion chromatography. A significant increase in immunogenicity measured by ELISA was observed in mice immunized with conjugated Pfs28 as compared to unconjugated Pfs28. Published by Elsevier Masson SAS.

U2 - 10.1016/j.micinf.2008.12.009

DO - 10.1016/j.micinf.2008.12.009

M3 - Article

VL - 11

SP - 408

EP - 412

JO - MICROBES AND INFECTION

JF - MICROBES AND INFECTION

IS - 3

ER -

Enhanced antibody responses to Plasmodium falciparum Pfs28 induced in mice by conjugation to ExoProtein A of Pseudomonas aeruginosa with an improved procedure

Abstract

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