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Enhanced bone tissue regeneration with hydrogel-based scaffolds by embedding parathyroid hormone in mesoporous bioactive glass

Research output: Contribution to journalArticlepeer-review

Mariane Beatriz Sordi, Márcio Celso Fredel, Ariadne Cristiane Cabral da Cruz, Paul Thomas Sharpe, Ricardo de Souza Magini

Original languageEnglish
JournalCLINICAL ORAL INVESTIGATIONS
DOIs
Accepted/In press18 Aug 2022
Published26 Aug 2022

Bibliographical note

Funding Information: M.B.S. received scholarship of the CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico) to perform this research. This project was partially funded by International Team for Implantology (ITI) Foundation, reference number 1113_2015. Publisher Copyright: © 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

King's Authors

Abstract

Objectives: To evaluate hydrogel-based scaffolds embedded with parathyroid hormone (PTH)-loaded mesoporous bioactive glass (MBG) on the enhancement of bone tissue regeneration in vitro. Materials and methods: MBG was produced via sol–gel technique followed by PTH solution imbibition. PTH-loaded MBG was blended into the hydrogels and submitted to a lyophilisation process associated with a chemical crosslinking reaction to the production of the scaffolds. Characterisation of the MBG and PTH-loaded MBG scaffolds, including the scanning electron microscope (SEM) connected with an X-ray detector (EDX), Fourier transform infrared (FTIR), compression strength, rheological measurements, swelling and degradation rates, and PTH release analysis, were performed. Also, bioactivity using simulated-body fluid (SBF), biocompatibility (MTT), and osteogenic differentiation analyses (von Kossa and Alizarin Red stainings, and μ-computed tomography, μCT) of the scaffolds were carried out. Results: SEM images demonstrated MBG particles dispersed into the hydrogel-based scaffold structure, which was homogeneously porous and well interconnected. EDX and FTIR revealed large amounts of carbon, oxygen, sodium, and silica in the scaffold composition. Bioactivity experiments revealed changes on sample surfaces over the analysed period, indicating the formation of carbonated hydroxyapatite; however, the chemical composition remained stable. PTH-loaded hydrogel-based scaffolds were biocompatible for stem cells from human-exfoliated deciduous teeth (SHED). A high quantity of calcium deposits on the extracellular matrix of SHED was found for PTH-loaded hydrogel-based scaffolds. μCT images showed MBG particles dispersed into the scaffolds’ structure, and a porous, lamellar, and interconnected hydrogel architecture. Conclusions: PTH-loaded hydrogel-based scaffolds demonstrated consistent morphology and physicochemical properties for bone tissue regeneration, as well as bioactivity, biocompatibility, and osteoinductivity in vitro. Thus, the scaffolds presented here are recommended for future studies on 3D printing. Clinical relevance: Bone tissue regeneration is still a challenge for several approaches to oral and maxillofacial surgeries, though tissue engineering applying SHED, scaffolds, and osteoinductive mediators might help to overcome this clinical issue.

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