Ensemble and single particle photophysical proper-ties (Two-Photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells

H  E Grecco; K  A Lidke; R Heintzmann; D  S Lidke; C Spagnuolo; O  E Martinez; E  A Jares-Erijman; T  M Jovin

doi:10.1002/jemt.20129

Ensemble and single particle photophysical proper-ties (Two-Photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells

H E Grecco, K A Lidke, R Heintzmann, D S Lidke, C Spagnuolo, O E Martinez, E A Jares-Erijman, T M Jovin

Research output: Contribution to journal › Article › peer-review

128 Citations (Scopus)

Abstract

In this work, we characterized streptavidin-conjugated quantum dots (QDs) manufactured by Quantum Dot Corporation. We present data on: (1) two-photon excitation; (2) fluorescence lifetimes; (3) ensemble and single QD emission anisotropy; (4) QDs as donors for Forster resonance energy transfer (FRET); and (5) spectral conversion of QDs exposed to high-intensity illumination. We also demonstrate the utility of QDs for (1) imaging the binding and uptake of biotinylated transferrin on living cells, and (2) resolving by fluorescence lifetime imaging microscopy (FLIM) signals originating from QDs from those of spatially and spectrally overlapping visible fluorescent proteins (VFPs). (C) 2005 Wiley-Liss. Inc.

Original language	English
Pages (from-to)	169 - 179
Number of pages	11
Journal	Microscopy Research and Technique
Volume	65
Issue number	4-5
DOIs	https://doi.org/10.1002/jemt.20129
Publication status	Published - Nov 2004

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Grecco, H. E., Lidke, K. A., Heintzmann, R., Lidke, D. S., Spagnuolo, C., Martinez, O. E., Jares-Erijman, E. A., & Jovin, T. M. (2004). Ensemble and single particle photophysical proper-ties (Two-Photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells. Microscopy Research and Technique, 65(4-5), 169 - 179. https://doi.org/10.1002/jemt.20129

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title = "Ensemble and single particle photophysical proper-ties (Two-Photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells",

abstract = "In this work, we characterized streptavidin-conjugated quantum dots (QDs) manufactured by Quantum Dot Corporation. We present data on: (1) two-photon excitation; (2) fluorescence lifetimes; (3) ensemble and single QD emission anisotropy; (4) QDs as donors for Forster resonance energy transfer (FRET); and (5) spectral conversion of QDs exposed to high-intensity illumination. We also demonstrate the utility of QDs for (1) imaging the binding and uptake of biotinylated transferrin on living cells, and (2) resolving by fluorescence lifetime imaging microscopy (FLIM) signals originating from QDs from those of spatially and spectrally overlapping visible fluorescent proteins (VFPs). (C) 2005 Wiley-Liss. Inc.",

author = "Grecco, {H E} and Lidke, {K A} and R Heintzmann and Lidke, {D S} and C Spagnuolo and Martinez, {O E} and Jares-Erijman, {E A} and Jovin, {T M}",

year = "2004",

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Grecco, HE, Lidke, KA, Heintzmann, R, Lidke, DS, Spagnuolo, C, Martinez, OE, Jares-Erijman, EA & Jovin, TM 2004, 'Ensemble and single particle photophysical proper-ties (Two-Photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells', Microscopy Research and Technique, vol. 65, no. 4-5, pp. 169 - 179. https://doi.org/10.1002/jemt.20129

Ensemble and single particle photophysical proper-ties (Two-Photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells. / Grecco, H E; Lidke, K A; Heintzmann, R et al.
In: Microscopy Research and Technique, Vol. 65, No. 4-5, 11.2004, p. 169 - 179.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Ensemble and single particle photophysical proper-ties (Two-Photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells

AU - Grecco, H E

AU - Lidke, K A

AU - Heintzmann, R

AU - Lidke, D S

AU - Spagnuolo, C

AU - Martinez, O E

AU - Jares-Erijman, E A

AU - Jovin, T M

PY - 2004/11

Y1 - 2004/11

N2 - In this work, we characterized streptavidin-conjugated quantum dots (QDs) manufactured by Quantum Dot Corporation. We present data on: (1) two-photon excitation; (2) fluorescence lifetimes; (3) ensemble and single QD emission anisotropy; (4) QDs as donors for Forster resonance energy transfer (FRET); and (5) spectral conversion of QDs exposed to high-intensity illumination. We also demonstrate the utility of QDs for (1) imaging the binding and uptake of biotinylated transferrin on living cells, and (2) resolving by fluorescence lifetime imaging microscopy (FLIM) signals originating from QDs from those of spatially and spectrally overlapping visible fluorescent proteins (VFPs). (C) 2005 Wiley-Liss. Inc.

AB - In this work, we characterized streptavidin-conjugated quantum dots (QDs) manufactured by Quantum Dot Corporation. We present data on: (1) two-photon excitation; (2) fluorescence lifetimes; (3) ensemble and single QD emission anisotropy; (4) QDs as donors for Forster resonance energy transfer (FRET); and (5) spectral conversion of QDs exposed to high-intensity illumination. We also demonstrate the utility of QDs for (1) imaging the binding and uptake of biotinylated transferrin on living cells, and (2) resolving by fluorescence lifetime imaging microscopy (FLIM) signals originating from QDs from those of spatially and spectrally overlapping visible fluorescent proteins (VFPs). (C) 2005 Wiley-Liss. Inc.

U2 - 10.1002/jemt.20129

DO - 10.1002/jemt.20129

M3 - Article

VL - 65

SP - 169

EP - 179

JO - Microscopy Research and Technique

JF - Microscopy Research and Technique

IS - 4-5

ER -

Ensemble and single particle photophysical proper-ties (Two-Photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells

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