ESCRT-III controls nuclear envelope reformation

TY - JOUR

T1 - ESCRT-III controls nuclear envelope reformation

AU - Olmos, Yolanda

AU - Hodgson, Lorna

AU - Mantell, Judith

AU - Verkade, Paul

AU - Carlton, Jeremy

N1 - Nature don't do OA, so this has also been deposited in Europe PMC.

PY - 2015/6/11

Y1 - 2015/6/11

N2 - During telophase, the nuclear envelope (NE) reforms around daughter nuclei to ensure proper segregation of nuclear and cytoplasmic contents1-4. NE reformation requires the coating of chromatin by membrane derived from the Endoplasmic Reticulum and a subsequent annular fusion step to ensure the formed envelope is sealed1,2,4,5. How annular fusion is accomplished is unknown, but it is thought to involve the p97 AAA-ATPase complex and bears a topological equivalence to the membrane fusion event that occurs during the abscission phase of cytokinesis1,6. We find here that the Endosomal Sorting Complex Required for Transport-III (ESCRT-III) machinery localises to sites of annular fusion in the forming NE and is necessary for proper post-mitotic nucleo-cytoplasmic compartmentalisation. The ESCRT-III component Charged Multivesicular Body Protein (CHMP) 2A is directed to the forming NE through binding to CHMP4B and provides an activity essential for NE reformation. Localisation also requires the p97 complex member Ubiquitin Fusion and Degradation 1 (UFD1). Our results describe a novel role for the ESCRT-machinery in cell division and demonstrate a conservation of the machineries involved in topologically equivalent mitotic membrane remodeling events.

AB - During telophase, the nuclear envelope (NE) reforms around daughter nuclei to ensure proper segregation of nuclear and cytoplasmic contents1-4. NE reformation requires the coating of chromatin by membrane derived from the Endoplasmic Reticulum and a subsequent annular fusion step to ensure the formed envelope is sealed1,2,4,5. How annular fusion is accomplished is unknown, but it is thought to involve the p97 AAA-ATPase complex and bears a topological equivalence to the membrane fusion event that occurs during the abscission phase of cytokinesis1,6. We find here that the Endosomal Sorting Complex Required for Transport-III (ESCRT-III) machinery localises to sites of annular fusion in the forming NE and is necessary for proper post-mitotic nucleo-cytoplasmic compartmentalisation. The ESCRT-III component Charged Multivesicular Body Protein (CHMP) 2A is directed to the forming NE through binding to CHMP4B and provides an activity essential for NE reformation. Localisation also requires the p97 complex member Ubiquitin Fusion and Degradation 1 (UFD1). Our results describe a novel role for the ESCRT-machinery in cell division and demonstrate a conservation of the machineries involved in topologically equivalent mitotic membrane remodeling events.

KW - Cell Biology

KW - ESCRT

KW - Nuclear Envelope

U2 - 10.1038/nature14503

DO - 10.1038/nature14503

M3 - Article

SN - 0028-0836

VL - 522

SP - 236

EP - 239

JO - NATURE

JF - NATURE

IS - 7555

ER -