Essential but sparse collagen hydroxylysyl post-translational modifications detected by DNP NMR

Wing Ying Chow; Rui Li; Ieva Goldberga; David G. Reid; Rakesh Rajan; Jonathan Clark; Hartmut Oschkinat; Melinda J. Duer; Robert Hayward; Catherine M. Shanahan

doi:10.1039/C8CC04960B

Essential but sparse collagen hydroxylysyl post-translational modifications detected by DNP NMR

Wing Ying Chow, Rui Li, Ieva Goldberga, David G. Reid, Rakesh Rajan, Jonathan Clark, Hartmut Oschkinat^*, Melinda J. Duer, Robert Hayward, Catherine M. Shanahan

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

The sparse but functionally essential post-translational collagen modification 5-hydroxylysine can undergo further transformations, including crosslinking, O-glycosylation, and glycation. Dynamic nuclear polarization (DNP) and stable isotope enriched lysine incorporation provide sufficient solid-state NMR sensitivity to identify these adducts directly in skin and vascular smooth muscle cell extracellular matrix (ECM), without extraction procedures, by comparison with chemical shifts of model compounds. Thus, DNP provides access to the elucidation of structural consequences of collagen modifications in intact tissue.

Original language	English
Pages (from-to)	12570-12573
Number of pages	4
Journal	Chemical Communications
Volume	54
Issue number	89
Early online date	5 Oct 2018
DOIs	https://doi.org/10.1039/C8CC04960B
Publication status	E-pub ahead of print - 5 Oct 2018

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title = "Essential but sparse collagen hydroxylysyl post-translational modifications detected by DNP NMR",

abstract = "The sparse but functionally essential post-translational collagen modification 5-hydroxylysine can undergo further transformations, including crosslinking, O-glycosylation, and glycation. Dynamic nuclear polarization (DNP) and stable isotope enriched lysine incorporation provide sufficient solid-state NMR sensitivity to identify these adducts directly in skin and vascular smooth muscle cell extracellular matrix (ECM), without extraction procedures, by comparison with chemical shifts of model compounds. Thus, DNP provides access to the elucidation of structural consequences of collagen modifications in intact tissue.",

author = "Chow, {Wing Ying} and Rui Li and Ieva Goldberga and Reid, {David G.} and Rakesh Rajan and Jonathan Clark and Hartmut Oschkinat and Duer, {Melinda J.} and Robert Hayward and Shanahan, {Catherine M.}",

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T1 - Essential but sparse collagen hydroxylysyl post-translational modifications detected by DNP NMR

AU - Chow, Wing Ying

AU - Li, Rui

AU - Goldberga, Ieva

AU - Reid, David G.

AU - Rajan, Rakesh

AU - Clark, Jonathan

AU - Oschkinat, Hartmut

AU - Duer, Melinda J.

AU - Hayward, Robert

AU - Shanahan, Catherine M.

PY - 2018/10/5

Y1 - 2018/10/5

N2 - The sparse but functionally essential post-translational collagen modification 5-hydroxylysine can undergo further transformations, including crosslinking, O-glycosylation, and glycation. Dynamic nuclear polarization (DNP) and stable isotope enriched lysine incorporation provide sufficient solid-state NMR sensitivity to identify these adducts directly in skin and vascular smooth muscle cell extracellular matrix (ECM), without extraction procedures, by comparison with chemical shifts of model compounds. Thus, DNP provides access to the elucidation of structural consequences of collagen modifications in intact tissue.

AB - The sparse but functionally essential post-translational collagen modification 5-hydroxylysine can undergo further transformations, including crosslinking, O-glycosylation, and glycation. Dynamic nuclear polarization (DNP) and stable isotope enriched lysine incorporation provide sufficient solid-state NMR sensitivity to identify these adducts directly in skin and vascular smooth muscle cell extracellular matrix (ECM), without extraction procedures, by comparison with chemical shifts of model compounds. Thus, DNP provides access to the elucidation of structural consequences of collagen modifications in intact tissue.

UR - http://www.scopus.com/inward/record.url?scp=85056088366&partnerID=8YFLogxK

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DO - 10.1039/C8CC04960B

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SN - 1359-7345

VL - 54

SP - 12570

EP - 12573

JO - Chemical Communications

JF - Chemical Communications

IS - 89

ER -

Essential but sparse collagen hydroxylysyl post-translational modifications detected by DNP NMR

Abstract

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