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Essential but sparse collagen hydroxylysyl post-translational modifications detected by DNP NMR

Research output: Contribution to journalArticle

Wing Ying Chow, Rui Li, Ieva Goldberga, David G. Reid, Rakesh Rajan, Jonathan Clark, Hartmut Oschkinat, Melinda J. Duer, Robert Hayward, Catherine M. Shanahan

Original languageEnglish
Pages (from-to)12570-12573
Number of pages4
JournalChemical Communications
Volume54
Issue number89
Early online date5 Oct 2018
DOIs
Publication statusE-pub ahead of print - 5 Oct 2018

King's Authors

Abstract

The sparse but functionally essential post-translational collagen modification 5-hydroxylysine can undergo further transformations, including crosslinking, O-glycosylation, and glycation. Dynamic nuclear polarization (DNP) and stable isotope enriched lysine incorporation provide sufficient solid-state NMR sensitivity to identify these adducts directly in skin and vascular smooth muscle cell extracellular matrix (ECM), without extraction procedures, by comparison with chemical shifts of model compounds. Thus, DNP provides access to the elucidation of structural consequences of collagen modifications in intact tissue.

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