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Essential but sparse collagen hydroxylysyl post-translational modifications detected by DNP NMR

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Essential but sparse collagen hydroxylysyl post-translational modifications detected by DNP NMR. / Chow, Wing Ying; Li, Rui; Goldberga, Ieva; Reid, David G.; Rajan, Rakesh; Clark, Jonathan; Oschkinat, Hartmut; Duer, Melinda J.; Hayward, Robert; Shanahan, Catherine M.

In: Chemical Communications, Vol. 54, No. 89, 05.10.2018, p. 12570-12573.

Research output: Contribution to journalArticle

Harvard

Chow, WY, Li, R, Goldberga, I, Reid, DG, Rajan, R, Clark, J, Oschkinat, H, Duer, MJ, Hayward, R & Shanahan, CM 2018, 'Essential but sparse collagen hydroxylysyl post-translational modifications detected by DNP NMR', Chemical Communications, vol. 54, no. 89, pp. 12570-12573. https://doi.org/10.1039/C8CC04960B

APA

Chow, W. Y., Li, R., Goldberga, I., Reid, D. G., Rajan, R., Clark, J., ... Shanahan, C. M. (2018). Essential but sparse collagen hydroxylysyl post-translational modifications detected by DNP NMR. Chemical Communications, 54(89), 12570-12573. https://doi.org/10.1039/C8CC04960B

Vancouver

Chow WY, Li R, Goldberga I, Reid DG, Rajan R, Clark J et al. Essential but sparse collagen hydroxylysyl post-translational modifications detected by DNP NMR. Chemical Communications. 2018 Oct 5;54(89):12570-12573. https://doi.org/10.1039/C8CC04960B

Author

Chow, Wing Ying ; Li, Rui ; Goldberga, Ieva ; Reid, David G. ; Rajan, Rakesh ; Clark, Jonathan ; Oschkinat, Hartmut ; Duer, Melinda J. ; Hayward, Robert ; Shanahan, Catherine M. / Essential but sparse collagen hydroxylysyl post-translational modifications detected by DNP NMR. In: Chemical Communications. 2018 ; Vol. 54, No. 89. pp. 12570-12573.

Bibtex Download

@article{985988cd09f644bfbc9b3680ae42e00c,
title = "Essential but sparse collagen hydroxylysyl post-translational modifications detected by DNP NMR",
abstract = "The sparse but functionally essential post-translational collagen modification 5-hydroxylysine can undergo further transformations, including crosslinking, O-glycosylation, and glycation. Dynamic nuclear polarization (DNP) and stable isotope enriched lysine incorporation provide sufficient solid-state NMR sensitivity to identify these adducts directly in skin and vascular smooth muscle cell extracellular matrix (ECM), without extraction procedures, by comparison with chemical shifts of model compounds. Thus, DNP provides access to the elucidation of structural consequences of collagen modifications in intact tissue.",
author = "Chow, {Wing Ying} and Rui Li and Ieva Goldberga and Reid, {David G.} and Rakesh Rajan and Jonathan Clark and Hartmut Oschkinat and Duer, {Melinda J.} and Robert Hayward and Shanahan, {Catherine M.}",
year = "2018",
month = "10",
day = "5",
doi = "10.1039/C8CC04960B",
language = "English",
volume = "54",
pages = "12570--12573",
journal = "Chemical communications (Cambridge, England)",
issn = "1359-7345",
number = "89",

}

RIS (suitable for import to EndNote) Download

TY - JOUR

T1 - Essential but sparse collagen hydroxylysyl post-translational modifications detected by DNP NMR

AU - Chow, Wing Ying

AU - Li, Rui

AU - Goldberga, Ieva

AU - Reid, David G.

AU - Rajan, Rakesh

AU - Clark, Jonathan

AU - Oschkinat, Hartmut

AU - Duer, Melinda J.

AU - Hayward, Robert

AU - Shanahan, Catherine M.

PY - 2018/10/5

Y1 - 2018/10/5

N2 - The sparse but functionally essential post-translational collagen modification 5-hydroxylysine can undergo further transformations, including crosslinking, O-glycosylation, and glycation. Dynamic nuclear polarization (DNP) and stable isotope enriched lysine incorporation provide sufficient solid-state NMR sensitivity to identify these adducts directly in skin and vascular smooth muscle cell extracellular matrix (ECM), without extraction procedures, by comparison with chemical shifts of model compounds. Thus, DNP provides access to the elucidation of structural consequences of collagen modifications in intact tissue.

AB - The sparse but functionally essential post-translational collagen modification 5-hydroxylysine can undergo further transformations, including crosslinking, O-glycosylation, and glycation. Dynamic nuclear polarization (DNP) and stable isotope enriched lysine incorporation provide sufficient solid-state NMR sensitivity to identify these adducts directly in skin and vascular smooth muscle cell extracellular matrix (ECM), without extraction procedures, by comparison with chemical shifts of model compounds. Thus, DNP provides access to the elucidation of structural consequences of collagen modifications in intact tissue.

UR - http://www.scopus.com/inward/record.url?scp=85056088366&partnerID=8YFLogxK

U2 - 10.1039/C8CC04960B

DO - 10.1039/C8CC04960B

M3 - Article

C2 - 30299444

AN - SCOPUS:85056088366

VL - 54

SP - 12570

EP - 12573

JO - Chemical communications (Cambridge, England)

JF - Chemical communications (Cambridge, England)

SN - 1359-7345

IS - 89

ER -

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