Estradiol prevented intestinal ischemia and reperfusion-induced changes in intestinal permeability and motility in male rats

TY - JOUR

T1 - Estradiol prevented intestinal ischemia and reperfusion-induced changes in intestinal permeability and motility in male rats

AU - Ricardo-da-Silva, Fernanda Yamamoto

AU - Fantozzi, Evelyn Thaís

AU - Rodrigues-Garbin, Sara

AU - Domingos, Helori Vanni

AU - Oliveira-Filho, Ricardo Martins

AU - Vargaftig, Bernardo Boris

AU - Riffo-Vasquez, Yanira

AU - Breithaupt-Faloppa, Ana Cristina

AU - Tavares-de-Lima, Wothan

N1 - Funding Information: Disclosure and consent for publication: The authors declare no conflicts of interest or consent for publication. This study was supported by the Fundac¸ão de Amparo à Pesquisa do Estado de São Paulo (Grant no. 2013/ 15291-0). Wothan Tavares-de-Lima is a fellow researcher at CNPq. Fernanda Yamamoto Ricardo da Silva was supported by CAPES. This study was financed, in part, by the Coordenac¸ão de Aperfeic¸oamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001. Funding Information: This study was supported by the Fundac??o de Amparo ? Pesquisa do Estado de S?o Paulo (Grant no. 2013/ 15291-0). Wothan Tavares-de-Lima is a fellow researcher at CNPq. Fernanda Yamamoto Ricardo da Silva was supported by CAPES. This study was financed, in part, by the Coordenac??o de Aperfeic?oamento de Pessoal de N?vel Superior-Brasil (CAPES)-Finance Code 001. Publisher Copyright: © 2021 CLINICS. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

PY - 2021

Y1 - 2021

N2 - OBJECTIVES: Ischemia and reperfusion (I/R) in the intestine could lead to severe endothelial injury, compromising intestinal motility. Reportedly, estradiol can control local and systemic inflammation induced by I/R injury. Thus, we investigated the effects of estradiol treatment on local repercussions in an intestinal I/R model. METHODS: Rats were subjected to ischemia via the occlusion of the superior mesenteric artery (45 min) followed by reperfusion (2h). Thirty minutes after ischemia induction (E30), 17b-estradiol (E2) was administered as a single dose (280 mg/kg, intravenous). Sham-operated animals were used as controls. RESULTS: I/R injury decreased intestinal motility and increased intestinal permeability, accompanied by reduced mesenteric endothelial nitric oxide synthase (eNOS) and endothelin (ET) protein expression. Additionally, the levels of serum injury markers and inflammatory mediators were elevated. Estradiol treatment improved intestinal motility, reduced intestinal permeability, and increased eNOS and ET expression. Levels of injury markers and inflammatory mediators were also reduced following estradiol treatment. CONCLUSION: Collectively, our findings indicate that estradiol treatment can modulate the deleterious intestinal effects of I/R injury. Thus, estradiol mediates the improvement in gut barrier functions and prevents intestinal dysfunction, which may reduce the systemic inflammatory response.

AB - OBJECTIVES: Ischemia and reperfusion (I/R) in the intestine could lead to severe endothelial injury, compromising intestinal motility. Reportedly, estradiol can control local and systemic inflammation induced by I/R injury. Thus, we investigated the effects of estradiol treatment on local repercussions in an intestinal I/R model. METHODS: Rats were subjected to ischemia via the occlusion of the superior mesenteric artery (45 min) followed by reperfusion (2h). Thirty minutes after ischemia induction (E30), 17b-estradiol (E2) was administered as a single dose (280 mg/kg, intravenous). Sham-operated animals were used as controls. RESULTS: I/R injury decreased intestinal motility and increased intestinal permeability, accompanied by reduced mesenteric endothelial nitric oxide synthase (eNOS) and endothelin (ET) protein expression. Additionally, the levels of serum injury markers and inflammatory mediators were elevated. Estradiol treatment improved intestinal motility, reduced intestinal permeability, and increased eNOS and ET expression. Levels of injury markers and inflammatory mediators were also reduced following estradiol treatment. CONCLUSION: Collectively, our findings indicate that estradiol treatment can modulate the deleterious intestinal effects of I/R injury. Thus, estradiol mediates the improvement in gut barrier functions and prevents intestinal dysfunction, which may reduce the systemic inflammatory response.

UR - http://www.scopus.com/inward/record.url?scp=85105065182&partnerID=8YFLogxK

U2 - 10.6061/clinics/2021/e2683

DO - 10.6061/clinics/2021/e2683

M3 - Article

C2 - 33909827

AN - SCOPUS:85105065182

SN - 1980-5322

VL - 76

SP - e2683

JO - Clinics (Sao Paulo, Brazil)

JF - Clinics (Sao Paulo, Brazil)

M1 - e2683

ER -