Ex vivo delivery of Mirococept: A dose finding study in pig kidney after showing a low dose is insufficient to reduce delayed graft function in human kidney

TY - JOUR

T1 - Ex vivo delivery of Mirococept: A dose finding study in pig kidney after showing a low dose is insufficient to reduce delayed graft function in human kidney

AU - Kassimatis, Theodoros

AU - Greenlaw, Roseanna

AU - Hunter, James P.

AU - Douiri, Abdel

AU - Flach, Clare

AU - Rebollo-Mesa, Irene

AU - Nichols, Laura L.

AU - Qasem, Anass

AU - Danzi, Guilherme

AU - Olsburgh, Jonathon

AU - Drage, Martin

AU - Friend, Peter J.

AU - Neri, Flavia

AU - Karegli, Julieta

AU - Horsfield, Catherine

AU - Smith, Richard A.

AU - Sacks, Steven H.

PY - 2020/8/17

Y1 - 2020/8/17

N2 - The complement system plays a pivotal role in the pathogenesis of ischemia–reperfusion injury in solid organ transplantation. Mirococept is a potent membrane-localizing complement inhibitor that can be administered ex vivo to the donor kidney prior to transplantation. To evaluate the efficacy of Mirococept in reducing delayed graft function (DGF) in deceased donor renal transplantation, we undertook the efficacy of mirococept (APT070) for preventing ischaemia-reperfusion injury in the kidney allograft (EMPIRIKAL) trial (ISRCTN49958194). A dose range of 5-25 mg would be tested, starting with 10 mg in cohort 1. No significant difference between Mirococept at 10 mg and control was detected; hence the study was stopped to enable a further dose saturation study in a porcine kidney model. The optimal dose of Mirococept in pig kidney was 80 mg. This dose did not induce any additional histological damage compared to controls or after a subsequent 3 hours of normothermic machine perfusion. The amount of unbound Mirococept postperfusion was found to be within the systemic dose range considered safe in the Phase I trial. The ex vivo administration of Mirococept is a safe and feasible approach to treat DGF in deceased donor kidney transplantation. The porcine kidney study identified an optimal dose of 80 mg (equivalent to 120 mg in human kidney) that provides a basis for further clinical development.

AB - The complement system plays a pivotal role in the pathogenesis of ischemia–reperfusion injury in solid organ transplantation. Mirococept is a potent membrane-localizing complement inhibitor that can be administered ex vivo to the donor kidney prior to transplantation. To evaluate the efficacy of Mirococept in reducing delayed graft function (DGF) in deceased donor renal transplantation, we undertook the efficacy of mirococept (APT070) for preventing ischaemia-reperfusion injury in the kidney allograft (EMPIRIKAL) trial (ISRCTN49958194). A dose range of 5-25 mg would be tested, starting with 10 mg in cohort 1. No significant difference between Mirococept at 10 mg and control was detected; hence the study was stopped to enable a further dose saturation study in a porcine kidney model. The optimal dose of Mirococept in pig kidney was 80 mg. This dose did not induce any additional histological damage compared to controls or after a subsequent 3 hours of normothermic machine perfusion. The amount of unbound Mirococept postperfusion was found to be within the systemic dose range considered safe in the Phase I trial. The ex vivo administration of Mirococept is a safe and feasible approach to treat DGF in deceased donor kidney transplantation. The porcine kidney study identified an optimal dose of 80 mg (equivalent to 120 mg in human kidney) that provides a basis for further clinical development.

KW - animal models: porcine

KW - clinical research/practice

KW - complement biology

KW - ischemia reperfusion injury (IRI)

KW - kidney transplantation/nephrology

KW - translational research/science

UR - http://www.scopus.com/inward/record.url?scp=85091791616&partnerID=8YFLogxK

U2 - 10.1111/ajt.16265

DO - 10.1111/ajt.16265

M3 - Article

SN - 1600-6135

JO - American Journal of Transplantation

JF - American Journal of Transplantation

ER -