Experimental approaches for gene regulatory network construction: the chick as a model system

TY - JOUR

T1 - Experimental approaches for gene regulatory network construction

T2 - the chick as a model system

AU - Streit, Andrea

AU - Tambalo, Monica

AU - Chen, Jingchen

AU - Grocott, Timothy

AU - Anwar, Maryam

AU - Sosinsky, Alona

AU - Stern, Claudio D

PY - 2013/5

Y1 - 2013/5

N2 - Setting up the body plan during embryonic development requires the coordinated action of many signals and transcriptional regulators in a precise temporal sequence and spatial pattern. The last decades have seen an explosion of information describing the molecular control of many developmental processes. The next challenge is to integrate this information into logic "wiring diagrams" that visualize gene actions and outputs, have predictive power and point to key control nodes. Here, we provide an experimental workflow on how to construct gene regulatory networks using the chick as model system.

AB - Setting up the body plan during embryonic development requires the coordinated action of many signals and transcriptional regulators in a precise temporal sequence and spatial pattern. The last decades have seen an explosion of information describing the molecular control of many developmental processes. The next challenge is to integrate this information into logic "wiring diagrams" that visualize gene actions and outputs, have predictive power and point to key control nodes. Here, we provide an experimental workflow on how to construct gene regulatory networks using the chick as model system.

KW - Animals

KW - Chick Embryo

KW - Chickens

KW - Enhancer Elements, Genetic

KW - Gene Expression Profiling

KW - Gene Expression Regulation

KW - Gene Regulatory Networks

U2 - 10.1002/dvg.22359

DO - 10.1002/dvg.22359

M3 - Article

C2 - 23174848

SN - 1526-968X

VL - 51

SP - 296

EP - 310

JO - Genesis (New York, N.Y. : 2000)

JF - Genesis (New York, N.Y. : 2000)

IS - 5

ER -