Expression of bacterial isochorismate synthase (EC 5.4.99.6) in transgenic root cultures of Rubia peregrina

A. H. Lodhi; R. J.M. Bongaerts; R. Verpoorte; S. A. Coomber; B. V. Charlwood

doi:10.1007/BF01275449

Expression of bacterial isochorismate synthase (EC 5.4.99.6) in transgenic root cultures of Rubia peregrina

A. H. Lodhi, R. J.M. Bongaerts, R. Verpoorte, S. A. Coomber, B. V. Charlwood^*

^*Corresponding author for this work

King's College London

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

The gene coding for isochorismate synthase (EC 5.4.99.6) was amplified by the polymerase chain reaction from Escherichia coli. cloned into a binary vector and mobilised into Agrobacterium rhizogenes LBA9402 which was used to transform Rubia peregrina. Transgenic roots containing bacterial isochorismate synthase cDNA expressed twice as much isochorismate synthase activity (4.88 pkat/mg protein) as the control roots (2.45 pkat/mg protein) after 10 days in culture, and accumulated ca. 20% higher levels of total anthraquinones after 30 days in culture. Whilst the amount of total alizarin (free and bound) in the transgenic roots was 30% higher than in control roots, the level of free alizarin was ca. 85% higher.

Original language	English
Pages (from-to)	54-57
Number of pages	4
Journal	Plant Cell Reports
Volume	16
Issue number	1-2
Early online date	Nov 1996
DOIs	https://doi.org/10.1007/BF01275449
Publication status	E-pub ahead of print - Nov 1996

Keywords

alizarin production
isochorismate synthase
Rubia peregrina
transgenic roots

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10.1007/BF01275449

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title = "Expression of bacterial isochorismate synthase (EC 5.4.99.6) in transgenic root cultures of Rubia peregrina",

abstract = "The gene coding for isochorismate synthase (EC 5.4.99.6) was amplified by the polymerase chain reaction from Escherichia coli. cloned into a binary vector and mobilised into Agrobacterium rhizogenes LBA9402 which was used to transform Rubia peregrina. Transgenic roots containing bacterial isochorismate synthase cDNA expressed twice as much isochorismate synthase activity (4.88 pkat/mg protein) as the control roots (2.45 pkat/mg protein) after 10 days in culture, and accumulated ca. 20% higher levels of total anthraquinones after 30 days in culture. Whilst the amount of total alizarin (free and bound) in the transgenic roots was 30% higher than in control roots, the level of free alizarin was ca. 85% higher.",

keywords = "alizarin production, isochorismate synthase, Rubia peregrina, transgenic roots",

author = "Lodhi, {A. H.} and Bongaerts, {R. J.M.} and R. Verpoorte and Coomber, {S. A.} and Charlwood, {B. V.}",

year = "1996",

month = nov,

doi = "10.1007/BF01275449",

language = "English",

volume = "16",

pages = "54--57",

journal = "Plant Cell Reports",

issn = "0721-7714",

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TY - JOUR

T1 - Expression of bacterial isochorismate synthase (EC 5.4.99.6) in transgenic root cultures of Rubia peregrina

AU - Lodhi, A. H.

AU - Bongaerts, R. J.M.

AU - Verpoorte, R.

AU - Coomber, S. A.

AU - Charlwood, B. V.

PY - 1996/11

Y1 - 1996/11

N2 - The gene coding for isochorismate synthase (EC 5.4.99.6) was amplified by the polymerase chain reaction from Escherichia coli. cloned into a binary vector and mobilised into Agrobacterium rhizogenes LBA9402 which was used to transform Rubia peregrina. Transgenic roots containing bacterial isochorismate synthase cDNA expressed twice as much isochorismate synthase activity (4.88 pkat/mg protein) as the control roots (2.45 pkat/mg protein) after 10 days in culture, and accumulated ca. 20% higher levels of total anthraquinones after 30 days in culture. Whilst the amount of total alizarin (free and bound) in the transgenic roots was 30% higher than in control roots, the level of free alizarin was ca. 85% higher.

AB - The gene coding for isochorismate synthase (EC 5.4.99.6) was amplified by the polymerase chain reaction from Escherichia coli. cloned into a binary vector and mobilised into Agrobacterium rhizogenes LBA9402 which was used to transform Rubia peregrina. Transgenic roots containing bacterial isochorismate synthase cDNA expressed twice as much isochorismate synthase activity (4.88 pkat/mg protein) as the control roots (2.45 pkat/mg protein) after 10 days in culture, and accumulated ca. 20% higher levels of total anthraquinones after 30 days in culture. Whilst the amount of total alizarin (free and bound) in the transgenic roots was 30% higher than in control roots, the level of free alizarin was ca. 85% higher.

KW - alizarin production

KW - isochorismate synthase

KW - Rubia peregrina

KW - transgenic roots

U2 - 10.1007/BF01275449

DO - 10.1007/BF01275449

M3 - Article

AN - SCOPUS:0030471819

SN - 0721-7714

VL - 16

SP - 54

EP - 57

JO - Plant Cell Reports

JF - Plant Cell Reports

IS - 1-2

ER -

Expression of bacterial isochorismate synthase (EC 5.4.99.6) in transgenic root cultures of Rubia peregrina

Abstract

Keywords

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