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Expression of catechol-O-methyltransferase in the brain and periphery of normal and MPTP-treated common marmosets

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Expression of catechol-O-methyltransferase in the brain and periphery of normal and MPTP-treated common marmosets. / Zeng, Bai-Yun; Balfour, Robert H.; Jackson, Mike J.; Rose, Sarah; Jenner, Peter.

In: Journal of Neural Transmission, Vol. 117, No. 1, 01.2010, p. 45 - 51.

Research output: Contribution to journalArticle

Harvard

Zeng, B-Y, Balfour, RH, Jackson, MJ, Rose, S & Jenner, P 2010, 'Expression of catechol-O-methyltransferase in the brain and periphery of normal and MPTP-treated common marmosets', Journal of Neural Transmission, vol. 117, no. 1, pp. 45 - 51. https://doi.org/10.1007/s00702-009-0315-9

APA

Zeng, B-Y., Balfour, R. H., Jackson, M. J., Rose, S., & Jenner, P. (2010). Expression of catechol-O-methyltransferase in the brain and periphery of normal and MPTP-treated common marmosets. Journal of Neural Transmission, 117(1), 45 - 51. https://doi.org/10.1007/s00702-009-0315-9

Vancouver

Zeng B-Y, Balfour RH, Jackson MJ, Rose S, Jenner P. Expression of catechol-O-methyltransferase in the brain and periphery of normal and MPTP-treated common marmosets. Journal of Neural Transmission. 2010 Jan;117(1):45 - 51. https://doi.org/10.1007/s00702-009-0315-9

Author

Zeng, Bai-Yun ; Balfour, Robert H. ; Jackson, Mike J. ; Rose, Sarah ; Jenner, Peter. / Expression of catechol-O-methyltransferase in the brain and periphery of normal and MPTP-treated common marmosets. In: Journal of Neural Transmission. 2010 ; Vol. 117, No. 1. pp. 45 - 51.

Bibtex Download

@article{2c429d48bfd145d8893feb6a5d10f10a,
title = "Expression of catechol-O-methyltransferase in the brain and periphery of normal and MPTP-treated common marmosets",
abstract = "Catechol-O-methyltransferase (COMT) inhibition is widely used to potentiate the effects of levodopa in Parkinson's disease but the effects of nigral dopaminergic cell loss and levodopa treatment on COMT activity are not known. The present study investigated the expression of COMT in the brain and liver of normal common marmosets, and animals treated with MPTP and those treated with levodopa to induce dyskinesia. Reverse transcript PCR demonstrated the expression of COMT mRNA in the liver, cortex and striatum of normal marmosets. Using Western blotting, the presence of two subunits of COMT protein, membrane bound COMT (MB-COMT) and soluble COMT (S-COMT), was shown in the liver, cortex and striatum of normal marmosets. Quantitative analysis of the MB-COMT and S-COMT subunit bands showed that there was no significant difference in the density of bands in MPTP treated marmosets or those exposed to levodopa compared to normal animals. COMT immunoreactivity was expressed in many brain regions including the cortex and striatum. No difference in COMT staining intensity was observed between normal, MPTP exposed or MPTP plus levodopa treated animals. COMT immunostaining was present in most striatal neurones and it was occasionally seen in glial cells. The data from present study demonstrated the expression of COMT mRNA and protein in the brain of common marmoset contrary to a previous report that it is not expressed in this species. COMT activity appears unaffected by loss of the dopaminergic nigro-striatal pathway and levodopa treatment.",
author = "Bai-Yun Zeng and Balfour, {Robert H.} and Jackson, {Mike J.} and Sarah Rose and Peter Jenner",
year = "2010",
month = "1",
doi = "10.1007/s00702-009-0315-9",
language = "English",
volume = "117",
pages = "45 -- 51",
journal = "Journal of Neural Transmission",
issn = "0300-9564",
number = "1",

}

RIS (suitable for import to EndNote) Download

TY - JOUR

T1 - Expression of catechol-O-methyltransferase in the brain and periphery of normal and MPTP-treated common marmosets

AU - Zeng, Bai-Yun

AU - Balfour, Robert H.

AU - Jackson, Mike J.

AU - Rose, Sarah

AU - Jenner, Peter

PY - 2010/1

Y1 - 2010/1

N2 - Catechol-O-methyltransferase (COMT) inhibition is widely used to potentiate the effects of levodopa in Parkinson's disease but the effects of nigral dopaminergic cell loss and levodopa treatment on COMT activity are not known. The present study investigated the expression of COMT in the brain and liver of normal common marmosets, and animals treated with MPTP and those treated with levodopa to induce dyskinesia. Reverse transcript PCR demonstrated the expression of COMT mRNA in the liver, cortex and striatum of normal marmosets. Using Western blotting, the presence of two subunits of COMT protein, membrane bound COMT (MB-COMT) and soluble COMT (S-COMT), was shown in the liver, cortex and striatum of normal marmosets. Quantitative analysis of the MB-COMT and S-COMT subunit bands showed that there was no significant difference in the density of bands in MPTP treated marmosets or those exposed to levodopa compared to normal animals. COMT immunoreactivity was expressed in many brain regions including the cortex and striatum. No difference in COMT staining intensity was observed between normal, MPTP exposed or MPTP plus levodopa treated animals. COMT immunostaining was present in most striatal neurones and it was occasionally seen in glial cells. The data from present study demonstrated the expression of COMT mRNA and protein in the brain of common marmoset contrary to a previous report that it is not expressed in this species. COMT activity appears unaffected by loss of the dopaminergic nigro-striatal pathway and levodopa treatment.

AB - Catechol-O-methyltransferase (COMT) inhibition is widely used to potentiate the effects of levodopa in Parkinson's disease but the effects of nigral dopaminergic cell loss and levodopa treatment on COMT activity are not known. The present study investigated the expression of COMT in the brain and liver of normal common marmosets, and animals treated with MPTP and those treated with levodopa to induce dyskinesia. Reverse transcript PCR demonstrated the expression of COMT mRNA in the liver, cortex and striatum of normal marmosets. Using Western blotting, the presence of two subunits of COMT protein, membrane bound COMT (MB-COMT) and soluble COMT (S-COMT), was shown in the liver, cortex and striatum of normal marmosets. Quantitative analysis of the MB-COMT and S-COMT subunit bands showed that there was no significant difference in the density of bands in MPTP treated marmosets or those exposed to levodopa compared to normal animals. COMT immunoreactivity was expressed in many brain regions including the cortex and striatum. No difference in COMT staining intensity was observed between normal, MPTP exposed or MPTP plus levodopa treated animals. COMT immunostaining was present in most striatal neurones and it was occasionally seen in glial cells. The data from present study demonstrated the expression of COMT mRNA and protein in the brain of common marmoset contrary to a previous report that it is not expressed in this species. COMT activity appears unaffected by loss of the dopaminergic nigro-striatal pathway and levodopa treatment.

U2 - 10.1007/s00702-009-0315-9

DO - 10.1007/s00702-009-0315-9

M3 - Article

VL - 117

SP - 45

EP - 51

JO - Journal of Neural Transmission

JF - Journal of Neural Transmission

SN - 0300-9564

IS - 1

ER -

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