Expression of Cre Recombinase in the developing mouse limb bud driven by a Prxl enhancer

Malcolm Logan; James F Martin; Andras Nagy; Corrinne Lobe; Eric N Olson; Clifford J Tabin

doi:10.1002/gene.10092

Expression of Cre Recombinase in the developing mouse limb bud driven by a Prxl enhancer

Malcolm Logan, James F Martin, Andras Nagy, Corrinne Lobe, Eric N Olson, Clifford J Tabin

Research output: Contribution to journal › Article › peer-review

786 Citations (Scopus)

Abstract

We have used a Prx1 limb enhancer to drive expression of Cre Recombinase in transgenic mice. This regulatory element leads to Cre expression throughout the early limb bud mesenchyme and in a subset of craniofacial mesenchyme. Crossing a murine line carrying this transgene to a reporter mouse harboring a floxed Cre-reporter cassette revealed that recombinase activity is first observed in the earliest limb bud at 9.5 dpc. By early to mid bud stages at 10.5 dpc recombination is essentially complete in all mesenchymal cells in the limb. Expression of the Cre recombinase was never detected in the limb bud ectoderm. The use of Prx1-Cre mice should facilitate analysis of gene function in the developing limb.

Original language	English
Pages (from-to)	77-80
Number of pages	4
Journal	GENESIS
Volume	33
Issue number	2
DOIs	https://doi.org/10.1002/gene.10092
Publication status	Published - Jun 2002

Keywords

Animals
Enhancer Elements, Genetic
Homeodomain Proteins/genetics
Integrases/genetics
Limb Buds
Mice
Mice, Transgenic
Viral Proteins/genetics

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10.1002/gene.10092

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title = "Expression of Cre Recombinase in the developing mouse limb bud driven by a Prxl enhancer",

abstract = "We have used a Prx1 limb enhancer to drive expression of Cre Recombinase in transgenic mice. This regulatory element leads to Cre expression throughout the early limb bud mesenchyme and in a subset of craniofacial mesenchyme. Crossing a murine line carrying this transgene to a reporter mouse harboring a floxed Cre-reporter cassette revealed that recombinase activity is first observed in the earliest limb bud at 9.5 dpc. By early to mid bud stages at 10.5 dpc recombination is essentially complete in all mesenchymal cells in the limb. Expression of the Cre recombinase was never detected in the limb bud ectoderm. The use of Prx1-Cre mice should facilitate analysis of gene function in the developing limb.",

keywords = "Animals, Enhancer Elements, Genetic, Homeodomain Proteins/genetics, Integrases/genetics, Limb Buds, Mice, Mice, Transgenic, Viral Proteins/genetics",

author = "Malcolm Logan and Martin, {James F} and Andras Nagy and Corrinne Lobe and Olson, {Eric N} and Tabin, {Clifford J}",

year = "2002",

month = jun,

doi = "10.1002/gene.10092",

language = "English",

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journal = "GENESIS",

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T1 - Expression of Cre Recombinase in the developing mouse limb bud driven by a Prxl enhancer

AU - Logan, Malcolm

AU - Martin, James F

AU - Nagy, Andras

AU - Lobe, Corrinne

AU - Olson, Eric N

AU - Tabin, Clifford J

PY - 2002/6

Y1 - 2002/6

N2 - We have used a Prx1 limb enhancer to drive expression of Cre Recombinase in transgenic mice. This regulatory element leads to Cre expression throughout the early limb bud mesenchyme and in a subset of craniofacial mesenchyme. Crossing a murine line carrying this transgene to a reporter mouse harboring a floxed Cre-reporter cassette revealed that recombinase activity is first observed in the earliest limb bud at 9.5 dpc. By early to mid bud stages at 10.5 dpc recombination is essentially complete in all mesenchymal cells in the limb. Expression of the Cre recombinase was never detected in the limb bud ectoderm. The use of Prx1-Cre mice should facilitate analysis of gene function in the developing limb.

AB - We have used a Prx1 limb enhancer to drive expression of Cre Recombinase in transgenic mice. This regulatory element leads to Cre expression throughout the early limb bud mesenchyme and in a subset of craniofacial mesenchyme. Crossing a murine line carrying this transgene to a reporter mouse harboring a floxed Cre-reporter cassette revealed that recombinase activity is first observed in the earliest limb bud at 9.5 dpc. By early to mid bud stages at 10.5 dpc recombination is essentially complete in all mesenchymal cells in the limb. Expression of the Cre recombinase was never detected in the limb bud ectoderm. The use of Prx1-Cre mice should facilitate analysis of gene function in the developing limb.

KW - Animals

KW - Enhancer Elements, Genetic

KW - Homeodomain Proteins/genetics

KW - Integrases/genetics

KW - Limb Buds

KW - Mice

KW - Mice, Transgenic

KW - Viral Proteins/genetics

U2 - 10.1002/gene.10092

DO - 10.1002/gene.10092

M3 - Article

C2 - 12112875

SN - 1526-954X

VL - 33

SP - 77

EP - 80

JO - GENESIS

JF - GENESIS

IS - 2

ER -

Expression of Cre Recombinase in the developing mouse limb bud driven by a Prxl enhancer

Abstract

Keywords

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