TY - JOUR
T1 - Expression of the guanine nucleotide exchange factor, RAPGEF5, during mouse and human embryogenesis
AU - Alharatani, Reham
AU - Griffin, John N.
AU - Liu, Karen J.
PY - 2019/12
Y1 - 2019/12
N2 - Rap GTPases mediate fundamental cellular processes, including cell adhesion, migration and intracellular signal transduction. The subcellular activity of these GTPases is regulated by dedicated activators (guanine nucleotide exchange factors, GEFs) and deactivators (GTPase-activating proteins, GAPs). RAPGEF5 is a potent activator of Rap proteins and mutations in RAPGEF5 have been linked to both neurological disorders and congenital heart disease. In the frog model, Xenopus tropicalis, RAPGEF5 is a critical regulator of the canonical Wnt signalling pathway and is required for normal gastrulation and correct establishment of the left-right body axis. However, requirements for RAPGEF5 in other developmental contexts, and in mammalian embryogenesis in particular, remain undefined. Here, we describe RAPGEF5 mRNA expression patterns during mouse (E9.5 - E16.5) and human (Carnegie stage 21) development, as an initial step towards better understanding its developmental functions.
AB - Rap GTPases mediate fundamental cellular processes, including cell adhesion, migration and intracellular signal transduction. The subcellular activity of these GTPases is regulated by dedicated activators (guanine nucleotide exchange factors, GEFs) and deactivators (GTPase-activating proteins, GAPs). RAPGEF5 is a potent activator of Rap proteins and mutations in RAPGEF5 have been linked to both neurological disorders and congenital heart disease. In the frog model, Xenopus tropicalis, RAPGEF5 is a critical regulator of the canonical Wnt signalling pathway and is required for normal gastrulation and correct establishment of the left-right body axis. However, requirements for RAPGEF5 in other developmental contexts, and in mammalian embryogenesis in particular, remain undefined. Here, we describe RAPGEF5 mRNA expression patterns during mouse (E9.5 - E16.5) and human (Carnegie stage 21) development, as an initial step towards better understanding its developmental functions.
KW - Guanine nucleotide exchange factor
KW - RAP GTPase
KW - Rapgef5
KW - mRNA expression
UR - http://www.scopus.com/inward/record.url?scp=85068032115&partnerID=8YFLogxK
U2 - 10.1016/j.gep.2019.119057
DO - 10.1016/j.gep.2019.119057
M3 - Article
SN - 1567-133X
VL - 34
JO - Gene Expression Patterns
JF - Gene Expression Patterns
M1 - 119057
ER -
