Expression of the guanine nucleotide exchange factor, RAPGEF5, during mouse and human embryogenesis

TY - JOUR

T1 - Expression of the guanine nucleotide exchange factor, RAPGEF5, during mouse and human embryogenesis

AU - Alharatani, Reham

AU - Griffin, John N.

AU - Liu, Karen J.

PY - 2019/12

Y1 - 2019/12

N2 - Rap GTPases mediate fundamental cellular processes, including cell adhesion, migration and intracellular signal transduction. The subcellular activity of these GTPases is regulated by dedicated activators (guanine nucleotide exchange factors, GEFs) and deactivators (GTPase-activating proteins, GAPs). RAPGEF5 is a potent activator of Rap proteins and mutations in RAPGEF5 have been linked to both neurological disorders and congenital heart disease. In the frog model, Xenopus tropicalis, RAPGEF5 is a critical regulator of the canonical Wnt signalling pathway and is required for normal gastrulation and correct establishment of the left-right body axis. However, requirements for RAPGEF5 in other developmental contexts, and in mammalian embryogenesis in particular, remain undefined. Here, we describe RAPGEF5 mRNA expression patterns during mouse (E9.5 - E16.5) and human (Carnegie stage 21) development, as an initial step towards better understanding its developmental functions.

AB - Rap GTPases mediate fundamental cellular processes, including cell adhesion, migration and intracellular signal transduction. The subcellular activity of these GTPases is regulated by dedicated activators (guanine nucleotide exchange factors, GEFs) and deactivators (GTPase-activating proteins, GAPs). RAPGEF5 is a potent activator of Rap proteins and mutations in RAPGEF5 have been linked to both neurological disorders and congenital heart disease. In the frog model, Xenopus tropicalis, RAPGEF5 is a critical regulator of the canonical Wnt signalling pathway and is required for normal gastrulation and correct establishment of the left-right body axis. However, requirements for RAPGEF5 in other developmental contexts, and in mammalian embryogenesis in particular, remain undefined. Here, we describe RAPGEF5 mRNA expression patterns during mouse (E9.5 - E16.5) and human (Carnegie stage 21) development, as an initial step towards better understanding its developmental functions.

KW - Guanine nucleotide exchange factor

KW - RAP GTPase

KW - Rapgef5

KW - mRNA expression

UR - https://www.scopus.com/pages/publications/85068032115

U2 - 10.1016/j.gep.2019.119057

DO - 10.1016/j.gep.2019.119057

M3 - Article

SN - 1567-133X

VL - 34

JO - Gene Expression Patterns

JF - Gene Expression Patterns

M1 - 119057

ER -