Expression profiling of mouse subplate reveals a dynamic gene network and disease association with autism and schizophrenia

Anna Hoerder-Suabedissen; Franziska M. Oeschger; Michelle Krishnan; T. Grant Belgard; Wei Zhi Wang; Sheena Lee; Caleb Webber; Enrico Petretto; A. David Edwards; Zoltan Molnar

doi:10.1073/pnas.1218510110

Expression profiling of mouse subplate reveals a dynamic gene network and disease association with autism and schizophrenia

Anna Hoerder-Suabedissen^*, Franziska M. Oeschger, Michelle Krishnan, T. Grant Belgard, Wei Zhi Wang, Sheena Lee, Caleb Webber, Enrico Petretto, A. David Edwards, Zoltan Molnar

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

96 Citations (Scopus)

Abstract

The subplate zone is a highly dynamic transient sector of the developing cerebral cortex that contains some of the earliest generated neurons and the first functional synapses of the cerebral cortex. Subplate cells have important functions in early establishment and maturation of thalamocortical connections, as well as in the development of inhibitory cortical circuits in sensory areas. So far no role has been identified for cells in the subplate in the mature brain and disease association of the subplate-specific genes has not been analyzed systematically. Here we present gene expression evidence for distinct roles of the mouse subplate across development as well as unique molecular markers to extend the repertoire of subplate labels. Performing systematic comparisons between different ages (embryonic days 15 and 18, postnatal day 8, and adult), we reveal the dynamic and constant features of the markers labeling subplate cells during embryonic and early postnatal development and in the adult. This can be visualized using the online database of subplate gene expression at https://molnar.dpag.ox.ac.uk/subplate/. We also identify embryonic similarities in gene expression between the ventricular zones, intermediate zone, and subplate, and distinct postnatal similarities between subplate, layer 5, and layers 2/3. The genes expressed in a subplate-specific manner at some point during development show a statistically significant enrichment for association with autism spectrum disorders and schizophrenia. Our report emphasizes the importance of the study of transient features of the developing brain to better understand neurodevelopmental disorders.

Original language	English
Pages (from-to)	3555-3560
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	110
Issue number	9
DOIs	https://doi.org/10.1073/pnas.1218510110
Publication status	Published - 26 Feb 2013

Keywords

SPECTRUM DISORDERS
WHITE-MATTER
DEVELOPMENTAL HISTORY
Nxph4
SOMATOSENSORY CORTEX
interstitial white matter cells
THALAMOCORTICAL CONNECTIONS
DIFFERENTIATION
BRAIN
CORTICAL SUBPLATE
Tpd52l1
microarray
CEREBRAL-CORTEX
NEURONS
RNAseq

Access to Document

10.1073/pnas.1218510110

The developing brain
Azzopardi, D., Counsell, S., Edwards, D., Hajnal, J. & Rutherford, M.
MRC Medical Research Council
1/07/2012 → 28/02/2019
Project: Research

Cite this

Hoerder-Suabedissen, A., Oeschger, F. M., Krishnan, M., Belgard, T. G., Wang, W. Z., Lee, S., Webber, C., Petretto, E., Edwards, A. D., & Molnar, Z. (2013). Expression profiling of mouse subplate reveals a dynamic gene network and disease association with autism and schizophrenia. Proceedings of the National Academy of Sciences of the United States of America, 110(9), 3555-3560. https://doi.org/10.1073/pnas.1218510110

@article{9cc31df5565c4dfd9b624f2dac604264,

title = "Expression profiling of mouse subplate reveals a dynamic gene network and disease association with autism and schizophrenia",

abstract = "The subplate zone is a highly dynamic transient sector of the developing cerebral cortex that contains some of the earliest generated neurons and the first functional synapses of the cerebral cortex. Subplate cells have important functions in early establishment and maturation of thalamocortical connections, as well as in the development of inhibitory cortical circuits in sensory areas. So far no role has been identified for cells in the subplate in the mature brain and disease association of the subplate-specific genes has not been analyzed systematically. Here we present gene expression evidence for distinct roles of the mouse subplate across development as well as unique molecular markers to extend the repertoire of subplate labels. Performing systematic comparisons between different ages (embryonic days 15 and 18, postnatal day 8, and adult), we reveal the dynamic and constant features of the markers labeling subplate cells during embryonic and early postnatal development and in the adult. This can be visualized using the online database of subplate gene expression at https://molnar.dpag.ox.ac.uk/subplate/. We also identify embryonic similarities in gene expression between the ventricular zones, intermediate zone, and subplate, and distinct postnatal similarities between subplate, layer 5, and layers 2/3. The genes expressed in a subplate-specific manner at some point during development show a statistically significant enrichment for association with autism spectrum disorders and schizophrenia. Our report emphasizes the importance of the study of transient features of the developing brain to better understand neurodevelopmental disorders.",

keywords = "SPECTRUM DISORDERS, WHITE-MATTER, DEVELOPMENTAL HISTORY, Nxph4, SOMATOSENSORY CORTEX, interstitial white matter cells, THALAMOCORTICAL CONNECTIONS, DIFFERENTIATION, BRAIN, CORTICAL SUBPLATE, Tpd52l1, microarray, CEREBRAL-CORTEX, NEURONS, RNAseq",

author = "Anna Hoerder-Suabedissen and Oeschger, {Franziska M.} and Michelle Krishnan and Belgard, {T. Grant} and Wang, {Wei Zhi} and Sheena Lee and Caleb Webber and Enrico Petretto and Edwards, {A. David} and Zoltan Molnar",

year = "2013",

month = feb,

day = "26",

doi = "10.1073/pnas.1218510110",

language = "English",

volume = "110",

pages = "3555--3560",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

issn = "0027-8424",

publisher = "National Acad Sciences",

number = "9",

}

Hoerder-Suabedissen, A, Oeschger, FM, Krishnan, M, Belgard, TG, Wang, WZ, Lee, S, Webber, C, Petretto, E, Edwards, AD & Molnar, Z 2013, 'Expression profiling of mouse subplate reveals a dynamic gene network and disease association with autism and schizophrenia', Proceedings of the National Academy of Sciences of the United States of America, vol. 110, no. 9, pp. 3555-3560. https://doi.org/10.1073/pnas.1218510110

Expression profiling of mouse subplate reveals a dynamic gene network and disease association with autism and schizophrenia. / Hoerder-Suabedissen, Anna; Oeschger, Franziska M.; Krishnan, Michelle et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, No. 9, 26.02.2013, p. 3555-3560.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Expression profiling of mouse subplate reveals a dynamic gene network and disease association with autism and schizophrenia

AU - Hoerder-Suabedissen, Anna

AU - Oeschger, Franziska M.

AU - Krishnan, Michelle

AU - Belgard, T. Grant

AU - Wang, Wei Zhi

AU - Lee, Sheena

AU - Webber, Caleb

AU - Petretto, Enrico

AU - Edwards, A. David

AU - Molnar, Zoltan

PY - 2013/2/26

Y1 - 2013/2/26

N2 - The subplate zone is a highly dynamic transient sector of the developing cerebral cortex that contains some of the earliest generated neurons and the first functional synapses of the cerebral cortex. Subplate cells have important functions in early establishment and maturation of thalamocortical connections, as well as in the development of inhibitory cortical circuits in sensory areas. So far no role has been identified for cells in the subplate in the mature brain and disease association of the subplate-specific genes has not been analyzed systematically. Here we present gene expression evidence for distinct roles of the mouse subplate across development as well as unique molecular markers to extend the repertoire of subplate labels. Performing systematic comparisons between different ages (embryonic days 15 and 18, postnatal day 8, and adult), we reveal the dynamic and constant features of the markers labeling subplate cells during embryonic and early postnatal development and in the adult. This can be visualized using the online database of subplate gene expression at https://molnar.dpag.ox.ac.uk/subplate/. We also identify embryonic similarities in gene expression between the ventricular zones, intermediate zone, and subplate, and distinct postnatal similarities between subplate, layer 5, and layers 2/3. The genes expressed in a subplate-specific manner at some point during development show a statistically significant enrichment for association with autism spectrum disorders and schizophrenia. Our report emphasizes the importance of the study of transient features of the developing brain to better understand neurodevelopmental disorders.

AB - The subplate zone is a highly dynamic transient sector of the developing cerebral cortex that contains some of the earliest generated neurons and the first functional synapses of the cerebral cortex. Subplate cells have important functions in early establishment and maturation of thalamocortical connections, as well as in the development of inhibitory cortical circuits in sensory areas. So far no role has been identified for cells in the subplate in the mature brain and disease association of the subplate-specific genes has not been analyzed systematically. Here we present gene expression evidence for distinct roles of the mouse subplate across development as well as unique molecular markers to extend the repertoire of subplate labels. Performing systematic comparisons between different ages (embryonic days 15 and 18, postnatal day 8, and adult), we reveal the dynamic and constant features of the markers labeling subplate cells during embryonic and early postnatal development and in the adult. This can be visualized using the online database of subplate gene expression at https://molnar.dpag.ox.ac.uk/subplate/. We also identify embryonic similarities in gene expression between the ventricular zones, intermediate zone, and subplate, and distinct postnatal similarities between subplate, layer 5, and layers 2/3. The genes expressed in a subplate-specific manner at some point during development show a statistically significant enrichment for association with autism spectrum disorders and schizophrenia. Our report emphasizes the importance of the study of transient features of the developing brain to better understand neurodevelopmental disorders.

KW - SPECTRUM DISORDERS

KW - WHITE-MATTER

KW - DEVELOPMENTAL HISTORY

KW - Nxph4

KW - SOMATOSENSORY CORTEX

KW - interstitial white matter cells

KW - THALAMOCORTICAL CONNECTIONS

KW - DIFFERENTIATION

KW - BRAIN

KW - CORTICAL SUBPLATE

KW - Tpd52l1

KW - microarray

KW - CEREBRAL-CORTEX

KW - NEURONS

KW - RNAseq

U2 - 10.1073/pnas.1218510110

DO - 10.1073/pnas.1218510110

M3 - Article

C2 - 23401504

SN - 0027-8424

VL - 110

SP - 3555

EP - 3560

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 9

ER -

Expression profiling of mouse subplate reveals a dynamic gene network and disease association with autism and schizophrenia

Abstract

Keywords

Access to Document

Fingerprint

Projects

The developing brain

Cite this