@article{fe60e07715bb4b219b80a8e892bddd6f,
title = "Extracellular matrix assembly stress initiates Drosophila central nervous system morphogenesis",
abstract = "Forces controlling tissue morphogenesis are attributed to cellular-driven activities, and any role for extracellular matrix (ECM) is assumed to be passive. However, all polymer networks, including ECM, can develop autonomous stresses during their assembly. Here, we examine the morphogenetic function of an ECM before reaching homeostatic equilibrium by analyzing de novo ECM assembly during Drosophila ventral nerve cord (VNC) condensation. Asymmetric VNC shortening and a rapid decrease in surface area correlate with the exponential assembly of collagen IV (Col4) surrounding the tissue. Concomitantly, a transient developmentally induced Col4 gradient leads to coherent long-range flow of ECM, which equilibrates the Col4 network. Finite element analysis and perturbation of Col4 network formation through the generation of dominant Col4 mutations that affect assembly reveal that VNC morphodynamics is partially driven by a sudden increase in ECM-driven surface tension. These data suggest that ECM assembly stress and associated network instabilities can actively participate in tissue morphogenesis.",
keywords = "Drosophila, Basement Membrane, Collagen IV, Morphogenesis, Central Nervous System, Extracellular Matrix, Surface Tension, Embryonic Development",
author = "{Serna Morales}, Eduardo and {S{\'a}nchez S{\'a}nchez}, Besaiz and Stefania Marcotti and Angus Nichols and Anushka Bhargava and Anca Dragu and Liisa Hirvonen and Mar{\'i}a-del-Carmen D{\'i}az-de-la-Loza and Matyas Mink and Susan Cox and Emily Rayfield and Lee, {Rachel M} and Hobson, {Chad M} and Teng-Leong Chew and Brian Stramer",
note = "Funding Information: We would like to thank Nir Gov, Rachel Lennon, Roberto Mayor, Patrick Mesquida, and David Sherwood for advice on the manuscript and Welcome Bender for the parasegmental driver. This research was funded by the Wellcome Trust (grant number 107859/Z/15/Z ) (B.M.S. and B.J.S.-S.), the European Research Council (ERC) under the European Union{\textquoteright}s Horizon 2020 research and innovation program (grant agreement no. 681808 ) (B.M.S. and S.M.), and the Consejo Nacional de Ciencia y Tecnolog{\'i}a (CONACYT), M{\'e}xico (E.S.-M.). The Advanced Imaging Center at Janelia Research Campus is supported by the Howard Hughes Medical Institute and the Gordon and Betty Moore Foundation. For the purpose of open access, the author has applied a CC BY public copyright license to any Author Accepted Manuscript version arising from this submission. Funding Information: We would like to thank Nir Gov, Rachel Lennon, Roberto Mayor, Patrick Mesquida, and David Sherwood for advice on the manuscript and Welcome Bender for the parasegmental driver. This research was funded by the Wellcome Trust (grant number 107859/Z/15/Z) (B.M.S. and B.J.S.-S.), the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program (grant agreement no. 681808) (B.M.S. and S.M.), and the Consejo Nacional de Ciencia y Tecnolog{\'i}a (CONACYT), M{\'e}xico (E.S.-M.). The Advanced Imaging Center at Janelia Research Campus is supported by the Howard Hughes Medical Institute and the Gordon and Betty Moore Foundation. For the purpose of open access, the author has applied a CC BY public copyright license to any Author Accepted Manuscript version arising from this submission. Conceptualization, E.S.-M. B.J.S.-S. S.M. and B.M.S.; methodology, E.S.-M. B.J.S.-S. S.M. M.M. B.M.S. C.M.H. R.M.L. and T.-L.C.; investigation, E.S.-M. B.J.S.-S. S.M. A.B. A.D. L.M.H. M.-d.-C.D.-d.-l.-L. M.M. S.C. E.R. B.M.S. C.M.H. and R.M.L.; funding acquisition, B.M.S. and T.-L.C.; project administration, E.S.-M. and B.M.S.; writing – original draft, B.M.S.; writing – review & editing, E.S.-M. B.J.S.-S. S.M. and B.M.S. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2023 The Author(s)",
year = "2023",
month = apr,
day = "21",
doi = "10.1016/j.devcel.2023.03.019",
language = "English",
volume = "58",
pages = "825--835.e6",
journal = "Developmental Cell",
issn = "1534-5807",
publisher = "Cell Press",
number = "10",
}