Extracellular matrix couples the convergence movements of mesoderm and neural plate during the early stages of neurulation

TY - JOUR

T1 - Extracellular matrix couples the convergence movements of mesoderm and neural plate during the early stages of neurulation

AU - Araya, Claudio

AU - Carmona-Fontaine, Carlos

AU - Clarke, Jonathan D. W.

PY - 2016/5/1

Y1 - 2016/5/1

N2 - Background: During the initial stages zebrafish neurulation, neural plate cells undergo highly coordinated movements before they assemble into a multicellular solid neural rod. We have previously identified that the underlying mesoderm is critical to ensure such coordination and generate correct neural tube organization. However, how intertissue coordination is achieved in vivo during zebrafish neural tube morphogenesis is unknown. Results: In this work, we use quantitative live imaging to study the coordinated movements of neural ectoderm and mesoderm during dorsal tissue convergence. We show the extracellular matrix components laminin and fibronectin that lie between mesoderm and neural plate act to couple the movements of neural plate and mesoderm during early stages of neurulation and to maintain the close apposition of these two tissues. Conclusions: Our study highlights the importance of the extracellular matrix proteins laminin and libronectin in coupling the movements and spatial proximity of mesoderm and neuroectoderm during the morphogenetic movements of neurulation.

AB - Background: During the initial stages zebrafish neurulation, neural plate cells undergo highly coordinated movements before they assemble into a multicellular solid neural rod. We have previously identified that the underlying mesoderm is critical to ensure such coordination and generate correct neural tube organization. However, how intertissue coordination is achieved in vivo during zebrafish neural tube morphogenesis is unknown. Results: In this work, we use quantitative live imaging to study the coordinated movements of neural ectoderm and mesoderm during dorsal tissue convergence. We show the extracellular matrix components laminin and fibronectin that lie between mesoderm and neural plate act to couple the movements of neural plate and mesoderm during early stages of neurulation and to maintain the close apposition of these two tissues. Conclusions: Our study highlights the importance of the extracellular matrix proteins laminin and libronectin in coupling the movements and spatial proximity of mesoderm and neuroectoderm during the morphogenetic movements of neurulation.

KW - Extracellular matrix

KW - Morphogenesis

KW - Neurulation

KW - Zebrafish

UR - http://www.scopus.com/inward/record.url?scp=84962767643&partnerID=8YFLogxK

U2 - 10.1002/dvdy.24401

DO - 10.1002/dvdy.24401

M3 - Article

AN - SCOPUS:84962767643

SN - 1058-8388

VL - 245

SP - 580

EP - 589

JO - Developmental Dynamics

JF - Developmental Dynamics

IS - 5

ER -