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Fascin Regulates Nuclear Movement and Deformation in Migrating Cells

Research output: Contribution to journalArticle

Asier Jayo, Majid Malboubi, Susumu Antoku, Wakam Chang, Elena Ortiz-Zapater, Christopher Groen, Karin Pfisterer, Tina Tootle, Guillaume Charras, Gregg G. Gundersen, Maddy Parsons

Original languageEnglish
Pages (from-to)371-383
Number of pages13
JournalDevelopmental Cell
Volume38
Issue number4
Early online date22 Aug 2016
DOIs
Accepted/In press25 Jul 2016
E-pub ahead of print22 Aug 2016
Published22 Aug 2016

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Abstract

Summary Fascin is an F-actin-bundling protein shown to stabilize filopodia and regulate adhesion dynamics in migrating cells, and its expression is correlated with poor prognosis and increased metastatic potential in a number of cancers. Here, we identified the nuclear envelope protein nesprin-2 as a binding partner for fascin in a range of cell types in vitro and in vivo. Nesprin-2 interacts with fascin through a direct, F-actin-independent interaction, and this binding is distinct and separable from a role for fascin within filopodia at the cell periphery. Moreover, disrupting the interaction between fascin and nesprin-2 C-terminal domain leads to specific defects in F-actin coupling to the nuclear envelope, nuclear movement, and the ability of cells to deform their nucleus to invade through confined spaces. Together, our results uncover a role for fascin that operates independently of filopodia assembly to promote efficient cell migration and invasion.

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