Fate of Prominin-1 Expressing Dermal Papilla Cells During Homeostasis, Wound Healing and Wnt Activation

Grace Kaushal; Emanuel Rognoni; Beate M Lichtenberger; Ryan R Driskell; Kai Kretzschmar; Esther Hoste; Fiona M Watt

doi:10.1038/jid.2015.319

Fate of Prominin-1 Expressing Dermal Papilla Cells During Homeostasis, Wound Healing and Wnt Activation

Grace Kaushal, Emanuel Rognoni, Beate M Lichtenberger, Ryan R Driskell, Kai Kretzschmar, Esther Hoste, Fiona M Watt

Gene Therapy and Regenerative Medicine

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Abstract

Prominin-1/CD133 (Prom1) is expressed by fibroblasts in the dermal papilla (DP) of the hair follicle (HF). By examining endogenous Prom1 expression and expression of LacZ in the skin of Prom1CreERLacZ (Prom1(C-L)) mice, in which a CreER(T2)-IRES-nuclear LacZ cassette is knocked into the first ATG codon of Prom1, we confirmed that Prom1 is expressed in the DP of all developing HFs and also by postnatal anagen follicles. To analyse the fate of Prom1+ DP cells, we crossed Prom1(C-L) mice with Rosa26-CAG flox/stop/flox tdTomato reporter mice and applied 4-hydroxytamoxifen (4OHT) to back skin at postnatal day (P) 1 and P2. We detected tdTomato+ cells in approximately 50% of DPs. The proportion of labelled cells per DP increased between P5 and P63, while the total number of cells per DP declined. Following full thickness wounding, there was no migration of tdTomato-labelled cells out of the DP. When β-catenin was activated in Prom1+ DP cells there was an increase in the size of anagen and telogen DP, but the proportion of tdTomato-labelled cells did not increase. We conclude that Prom1+ DP cells do not contribute to dermal repair but are nevertheless capable of regulating DP size via β-catenin mediated intercellular communication.

Original language	English
Pages (from-to)	2926–2934
Journal	Journal of Investigative Dermatology
Volume	135
Issue number	12
Early online date	10 Sept 2015
DOIs	https://doi.org/10.1038/jid.2015.319
Publication status	E-pub ahead of print - 10 Sept 2015

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Fate of Prominin-1 Expressing_KAUSHAL_Accepted 19Aug2015_GOLD VoRFinal published version, 7.64 MBLicence: CC BY-NC-SA

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@article{5e1216bd6b094dd4ad3efb9e7420d458,

title = "Fate of Prominin-1 Expressing Dermal Papilla Cells During Homeostasis, Wound Healing and Wnt Activation",

abstract = "Prominin-1/CD133 (Prom1) is expressed by fibroblasts in the dermal papilla (DP) of the hair follicle (HF). By examining endogenous Prom1 expression and expression of LacZ in the skin of Prom1CreERLacZ (Prom1(C-L)) mice, in which a CreER(T2)-IRES-nuclear LacZ cassette is knocked into the first ATG codon of Prom1, we confirmed that Prom1 is expressed in the DP of all developing HFs and also by postnatal anagen follicles. To analyse the fate of Prom1+ DP cells, we crossed Prom1(C-L) mice with Rosa26-CAG flox/stop/flox tdTomato reporter mice and applied 4-hydroxytamoxifen (4OHT) to back skin at postnatal day (P) 1 and P2. We detected tdTomato+ cells in approximately 50% of DPs. The proportion of labelled cells per DP increased between P5 and P63, while the total number of cells per DP declined. Following full thickness wounding, there was no migration of tdTomato-labelled cells out of the DP. When β-catenin was activated in Prom1+ DP cells there was an increase in the size of anagen and telogen DP, but the proportion of tdTomato-labelled cells did not increase. We conclude that Prom1+ DP cells do not contribute to dermal repair but are nevertheless capable of regulating DP size via β-catenin mediated intercellular communication.",

author = "Grace Kaushal and Emanuel Rognoni and Lichtenberger, {Beate M} and Driskell, {Ryan R} and Kai Kretzschmar and Esther Hoste and Watt, {Fiona M}",

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AU - Kaushal, Grace

AU - Rognoni, Emanuel

AU - Lichtenberger, Beate M

AU - Driskell, Ryan R

AU - Kretzschmar, Kai

AU - Hoste, Esther

AU - Watt, Fiona M

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N2 - Prominin-1/CD133 (Prom1) is expressed by fibroblasts in the dermal papilla (DP) of the hair follicle (HF). By examining endogenous Prom1 expression and expression of LacZ in the skin of Prom1CreERLacZ (Prom1(C-L)) mice, in which a CreER(T2)-IRES-nuclear LacZ cassette is knocked into the first ATG codon of Prom1, we confirmed that Prom1 is expressed in the DP of all developing HFs and also by postnatal anagen follicles. To analyse the fate of Prom1+ DP cells, we crossed Prom1(C-L) mice with Rosa26-CAG flox/stop/flox tdTomato reporter mice and applied 4-hydroxytamoxifen (4OHT) to back skin at postnatal day (P) 1 and P2. We detected tdTomato+ cells in approximately 50% of DPs. The proportion of labelled cells per DP increased between P5 and P63, while the total number of cells per DP declined. Following full thickness wounding, there was no migration of tdTomato-labelled cells out of the DP. When β-catenin was activated in Prom1+ DP cells there was an increase in the size of anagen and telogen DP, but the proportion of tdTomato-labelled cells did not increase. We conclude that Prom1+ DP cells do not contribute to dermal repair but are nevertheless capable of regulating DP size via β-catenin mediated intercellular communication.

AB - Prominin-1/CD133 (Prom1) is expressed by fibroblasts in the dermal papilla (DP) of the hair follicle (HF). By examining endogenous Prom1 expression and expression of LacZ in the skin of Prom1CreERLacZ (Prom1(C-L)) mice, in which a CreER(T2)-IRES-nuclear LacZ cassette is knocked into the first ATG codon of Prom1, we confirmed that Prom1 is expressed in the DP of all developing HFs and also by postnatal anagen follicles. To analyse the fate of Prom1+ DP cells, we crossed Prom1(C-L) mice with Rosa26-CAG flox/stop/flox tdTomato reporter mice and applied 4-hydroxytamoxifen (4OHT) to back skin at postnatal day (P) 1 and P2. We detected tdTomato+ cells in approximately 50% of DPs. The proportion of labelled cells per DP increased between P5 and P63, while the total number of cells per DP declined. Following full thickness wounding, there was no migration of tdTomato-labelled cells out of the DP. When β-catenin was activated in Prom1+ DP cells there was an increase in the size of anagen and telogen DP, but the proportion of tdTomato-labelled cells did not increase. We conclude that Prom1+ DP cells do not contribute to dermal repair but are nevertheless capable of regulating DP size via β-catenin mediated intercellular communication.

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Fate of Prominin-1 Expressing Dermal Papilla Cells During Homeostasis, Wound Healing and Wnt Activation

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