Fibroblast state switching orchestrates dermal maturation and wound healing

Emanuel Rognoni, Angela Oliveira Pisco, Toru Hiratsuka, Kalle H. Sipilä, Julio M. Belmonte, Seyedeh Atefeh Mobasseri, Christina Philippeos, Rui Dilão, Fiona M. Watt*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

92 Citations (Scopus)
238 Downloads (Pure)


Murine dermis contains functionally and spatially distinct fibroblast lineages that cease to proliferate in early postnatal life. Here, we propose a model in which a negative feedback loop between extracellular matrix (ECM) deposition and fibroblast proliferation determines dermal architecture. Virtual-tissue simulations of our model faithfully recapitulate dermal maturation, predicting a loss of spatial segregation of fibroblast lineages and dictating that fibroblast migration is only required for wound healing. To test this, we performed in vivo live imaging of dermal fibroblasts, which revealed that homeostatic tissue architecture is achieved without active cell migration. In contrast, both fibroblast proliferation and migration are key determinants of tissue repair following wounding. The results show that tissue-scale coordination is driven by the interdependence of cell proliferation and ECM deposition, paving the way for identifying new therapeutic strategies to enhance skin regeneration.

Original languageEnglish
Article numbere8174
JournalMolecular Systems Biology
Issue number8
Early online date29 Aug 2018
Publication statusPublished - Aug 2018


  • dermis development
  • fibroblast states
  • mathematical modelling
  • tissue architecture
  • wound healing


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