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Fine-tuning the onset of myogenesis by homeobox proteins that interact with the Myf5 limb enhancer

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Fine-tuning the onset of myogenesis by homeobox proteins that interact with the Myf5 limb enhancer. / Daubas, Philippe; Duval, Nathalie; Bajard, Lola; Vives, Francina Langa; Robert, Benoît; Mankoo, Baljinder S.; Buckingham, Margaret.

In: Biology Open, Vol. 4, No. 12, 16.12.2015, p. 1614-1624.

Research output: Contribution to journalArticle

Harvard

Daubas, P, Duval, N, Bajard, L, Vives, FL, Robert, B, Mankoo, BS & Buckingham, M 2015, 'Fine-tuning the onset of myogenesis by homeobox proteins that interact with the Myf5 limb enhancer', Biology Open, vol. 4, no. 12, pp. 1614-1624. https://doi.org/10.1242/bio.014068

APA

Daubas, P., Duval, N., Bajard, L., Vives, F. L., Robert, B., Mankoo, B. S., & Buckingham, M. (2015). Fine-tuning the onset of myogenesis by homeobox proteins that interact with the Myf5 limb enhancer. Biology Open, 4(12), 1614-1624. https://doi.org/10.1242/bio.014068

Vancouver

Daubas P, Duval N, Bajard L, Vives FL, Robert B, Mankoo BS et al. Fine-tuning the onset of myogenesis by homeobox proteins that interact with the Myf5 limb enhancer. Biology Open. 2015 Dec 16;4(12):1614-1624. https://doi.org/10.1242/bio.014068

Author

Daubas, Philippe ; Duval, Nathalie ; Bajard, Lola ; Vives, Francina Langa ; Robert, Benoît ; Mankoo, Baljinder S. ; Buckingham, Margaret. / Fine-tuning the onset of myogenesis by homeobox proteins that interact with the Myf5 limb enhancer. In: Biology Open. 2015 ; Vol. 4, No. 12. pp. 1614-1624.

Bibtex Download

@article{5924ff0627f1429fb30402409e43f319,
title = "Fine-tuning the onset of myogenesis by homeobox proteins that interact with the Myf5 limb enhancer",
abstract = "Skeletal myogenesis in vertebrates is initiated at different sites of skeletal muscle formation during development, by activation of specific control elements of the myogenic regulatory genes. In the mouse embryo, Myf5 is the first myogenic determination gene to be expressed and its spatiotemporal regulation requires multiple enhancer sequences, extending over 120 kb upstream of the Mrf4-Myf5 locus. An enhancer, located at -57/-58 kb from Myf5, is responsible for its activation in myogenic cells derived from the hypaxial domain of the somite, that will form limb muscles. Pax3 and Six1/4 transcription factors are essential activators of this enhancer, acting on a 145-bp core element. Myogenic progenitor cells that will form the future muscle masses of the limbs express the factors necessary for Myf5 activation when they delaminate from the hypaxial dermomyotome and migrate into the forelimb bud, however they do not activate Myf5 and the myogenic programme until they have populated the prospective muscle masses. We show that Msx1 and Meox2 homeodomain-containing transcription factors bind in vitro and in vivo to specific sites in the 145-bp element, and are implicated in fine-tuning activation of Myf5 in the forelimb. Msx1, when bound between Pax and Six sites, prevents the binding of these key activators, thus inhibiting transcription of Myf5 and consequent premature myogenic differentiation. Meox2 is required for Myf5 activation at the onset of myogenesis via direct binding to other homeodomain sites in this sequence. Thus, these homeodomain factors, acting in addition to Pax3 and Six1/4, fine-tune the entry of progenitor cells into myogenesis at early stages of forelimb development.",
keywords = "Limb myogenesis, Meox2, Mouse embryo, Msx1, Myf5 transcription",
author = "Philippe Daubas and Nathalie Duval and Lola Bajard and Vives, {Francina Langa} and Beno{\^i}t Robert and Mankoo, {Baljinder S.} and Margaret Buckingham",
year = "2015",
month = "12",
day = "16",
doi = "10.1242/bio.014068",
language = "English",
volume = "4",
pages = "1614--1624",
journal = "Biology Open",
issn = "2046-6390",
publisher = "Company of Biologists Ltd",
number = "12",

}

RIS (suitable for import to EndNote) Download

TY - JOUR

T1 - Fine-tuning the onset of myogenesis by homeobox proteins that interact with the Myf5 limb enhancer

AU - Daubas, Philippe

AU - Duval, Nathalie

AU - Bajard, Lola

AU - Vives, Francina Langa

AU - Robert, Benoît

AU - Mankoo, Baljinder S.

AU - Buckingham, Margaret

PY - 2015/12/16

Y1 - 2015/12/16

N2 - Skeletal myogenesis in vertebrates is initiated at different sites of skeletal muscle formation during development, by activation of specific control elements of the myogenic regulatory genes. In the mouse embryo, Myf5 is the first myogenic determination gene to be expressed and its spatiotemporal regulation requires multiple enhancer sequences, extending over 120 kb upstream of the Mrf4-Myf5 locus. An enhancer, located at -57/-58 kb from Myf5, is responsible for its activation in myogenic cells derived from the hypaxial domain of the somite, that will form limb muscles. Pax3 and Six1/4 transcription factors are essential activators of this enhancer, acting on a 145-bp core element. Myogenic progenitor cells that will form the future muscle masses of the limbs express the factors necessary for Myf5 activation when they delaminate from the hypaxial dermomyotome and migrate into the forelimb bud, however they do not activate Myf5 and the myogenic programme until they have populated the prospective muscle masses. We show that Msx1 and Meox2 homeodomain-containing transcription factors bind in vitro and in vivo to specific sites in the 145-bp element, and are implicated in fine-tuning activation of Myf5 in the forelimb. Msx1, when bound between Pax and Six sites, prevents the binding of these key activators, thus inhibiting transcription of Myf5 and consequent premature myogenic differentiation. Meox2 is required for Myf5 activation at the onset of myogenesis via direct binding to other homeodomain sites in this sequence. Thus, these homeodomain factors, acting in addition to Pax3 and Six1/4, fine-tune the entry of progenitor cells into myogenesis at early stages of forelimb development.

AB - Skeletal myogenesis in vertebrates is initiated at different sites of skeletal muscle formation during development, by activation of specific control elements of the myogenic regulatory genes. In the mouse embryo, Myf5 is the first myogenic determination gene to be expressed and its spatiotemporal regulation requires multiple enhancer sequences, extending over 120 kb upstream of the Mrf4-Myf5 locus. An enhancer, located at -57/-58 kb from Myf5, is responsible for its activation in myogenic cells derived from the hypaxial domain of the somite, that will form limb muscles. Pax3 and Six1/4 transcription factors are essential activators of this enhancer, acting on a 145-bp core element. Myogenic progenitor cells that will form the future muscle masses of the limbs express the factors necessary for Myf5 activation when they delaminate from the hypaxial dermomyotome and migrate into the forelimb bud, however they do not activate Myf5 and the myogenic programme until they have populated the prospective muscle masses. We show that Msx1 and Meox2 homeodomain-containing transcription factors bind in vitro and in vivo to specific sites in the 145-bp element, and are implicated in fine-tuning activation of Myf5 in the forelimb. Msx1, when bound between Pax and Six sites, prevents the binding of these key activators, thus inhibiting transcription of Myf5 and consequent premature myogenic differentiation. Meox2 is required for Myf5 activation at the onset of myogenesis via direct binding to other homeodomain sites in this sequence. Thus, these homeodomain factors, acting in addition to Pax3 and Six1/4, fine-tune the entry of progenitor cells into myogenesis at early stages of forelimb development.

KW - Limb myogenesis

KW - Meox2

KW - Mouse embryo

KW - Msx1

KW - Myf5 transcription

UR - http://www.scopus.com/inward/record.url?scp=84982803950&partnerID=8YFLogxK

U2 - 10.1242/bio.014068

DO - 10.1242/bio.014068

M3 - Article

AN - SCOPUS:84982803950

VL - 4

SP - 1614

EP - 1624

JO - Biology Open

JF - Biology Open

SN - 2046-6390

IS - 12

ER -

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