Flexible targeting of ErbB dimers that drive tumorigenesis by using genetically engineered T cells

David M Davies, Julie Foster, Sjoukje J C Van Der Stegen, Ana C Parente-Pereira, Laura Chiapero-Stanke, George J Delinassios, Sophie E Burbridge, Vincent Kao, Zhe Liu, Leticia Bosshard-Carter, May C I Van Schalkwyk, Carol Box, Suzanne A Eccles, Stephen J Mather, Scott Wilkie, John Maher

Research output: Contribution to journalArticlepeer-review

88 Citations (Scopus)

Abstract

Pharmacological targeting of individual ErbB receptors elicits antitumor activity, but is frequently compromised by resistance leading to therapeutic failure. Here, we describe an immunotherapeutic approach that exploits prevalent and fundamental mechanisms by which aberrant upregulation of the ErbB network drives tumorigenesis. A chimeric antigen receptor named T1E28z was engineered, in which the promiscuous ErbB ligand, T1E, is fused to a CD28 + CD3ζ endodomain. Using a panel of ErbB-engineered 32D hematopoietic cells, we found that human T1E28z⁺ T cells are selectively activated by all ErbB1-based homodimers and heterodimers and by the potently mitogenic ErbB2/3 heterodimer. Owing to this flexible targeting capability, recognition and destruction of several tumor cell lines was achieved by T1E28⁺ T cells in vitro, comprising a wide diversity of ErbB receptor profiles and tumor origins. Furthermore, compelling antitumor activity was observed in mice bearing established xenografts, characterized either by ErbB1/2 or ErbB2/3 overexpression and representative of insidious or rapidly progressive tumor types. Together, these findings support the clinical development of a broadly applicable immunotherapeutic approach in which the propensity of solid tumors to dysregulate the extended ErbB network is targeted for therapeutic gain.
Original languageEnglish
Pages (from-to)565-576
Number of pages12
JournalMolecular Medicine
Volume18
Issue number1
Early online date17 Feb 2012
DOIs
Publication statusPublished - Apr 2012

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