Abstract
Acetylcholine-induced vasodilatation is impaired in animal models of insulin-dependent diabetes mellitus (IDDM), and may result from altered nitric oxide synthesis or release. The response to intraluminal flow, a more physiologically relevant stimulus for nitric oxide release, is unknown. This study examined flow-induced responses in isolated resistance arteries from male Sprague-Dawley control and streptozotocin-diabetic (45 mg/kg i.v., 4 week duration) rats. Mesenteric arteries (4-5th order) were dissected and cannulated on a pressure myograph (mean internal diameter +/- SEM at 40 mmHg, control 223 +/- 8, n = 9 vs diabetic 239 +/- 12 microm, n = 8, NS). Arteries were preconstricted with noradrenaline (1 micromol/l) and intraluminal pressure raised and maintained at 80 mmHg. Luminal flow was raised in incremental steps (0-1.27 microl/s). Arteries from control animals dilated to flow while arteries from diabetic animals constricted (% change in internal diameter +/- SEM at 0.79 microl/s: control 13.46 +/- 6.52, n = 9 vs diabetic -7.44 +/- 3.38%, n = 8; p
| Original language | English |
|---|---|
| Pages (from-to) | 34-39 |
| Number of pages | 6 |
| Journal | Diabetologia |
| Volume | 41 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 1998 |
Keywords
- Acetylcholine
- Animals
- Blood Flow Velocity
- Diabetes Mellitus, Experimental
- Intestine, Small
- Male
- Mesenteric Arteries
- Muscle, Smooth, Vascular
- NG-Nitroarginine Methyl Ester
- Norepinephrine
- Rats
- Rats, Sprague-Dawley
- Reference Values
- Stress, Mechanical
- Vasodilation
