Flow-induced dilatation in isolated resistance arteries from control and streptozotocin-diabetic rats

TY - JOUR

T1 - Flow-induced dilatation in isolated resistance arteries from control and streptozotocin-diabetic rats

AU - Tribe, Rachel

AU - Thomas, C R

AU - Poston, L

PY - 1998/1

Y1 - 1998/1

N2 - Acetylcholine-induced vasodilatation is impaired in animal models of insulin-dependent diabetes mellitus (IDDM), and may result from altered nitric oxide synthesis or release. The response to intraluminal flow, a more physiologically relevant stimulus for nitric oxide release, is unknown. This study examined flow-induced responses in isolated resistance arteries from male Sprague-Dawley control and streptozotocin-diabetic (45 mg/kg i.v., 4 week duration) rats. Mesenteric arteries (4-5th order) were dissected and cannulated on a pressure myograph (mean internal diameter +/- SEM at 40 mmHg, control 223 +/- 8, n = 9 vs diabetic 239 +/- 12 microm, n = 8, NS). Arteries were preconstricted with noradrenaline (1 micromol/l) and intraluminal pressure raised and maintained at 80 mmHg. Luminal flow was raised in incremental steps (0-1.27 microl/s). Arteries from control animals dilated to flow while arteries from diabetic animals constricted (% change in internal diameter +/- SEM at 0.79 microl/s: control 13.46 +/- 6.52, n = 9 vs diabetic -7.44 +/- 3.38%, n = 8; p

AB - Acetylcholine-induced vasodilatation is impaired in animal models of insulin-dependent diabetes mellitus (IDDM), and may result from altered nitric oxide synthesis or release. The response to intraluminal flow, a more physiologically relevant stimulus for nitric oxide release, is unknown. This study examined flow-induced responses in isolated resistance arteries from male Sprague-Dawley control and streptozotocin-diabetic (45 mg/kg i.v., 4 week duration) rats. Mesenteric arteries (4-5th order) were dissected and cannulated on a pressure myograph (mean internal diameter +/- SEM at 40 mmHg, control 223 +/- 8, n = 9 vs diabetic 239 +/- 12 microm, n = 8, NS). Arteries were preconstricted with noradrenaline (1 micromol/l) and intraluminal pressure raised and maintained at 80 mmHg. Luminal flow was raised in incremental steps (0-1.27 microl/s). Arteries from control animals dilated to flow while arteries from diabetic animals constricted (% change in internal diameter +/- SEM at 0.79 microl/s: control 13.46 +/- 6.52, n = 9 vs diabetic -7.44 +/- 3.38%, n = 8; p

KW - Acetylcholine

KW - Animals

KW - Blood Flow Velocity

KW - Diabetes Mellitus, Experimental

KW - Intestine, Small

KW - Male

KW - Mesenteric Arteries

KW - Muscle, Smooth, Vascular

KW - NG-Nitroarginine Methyl Ester

KW - Norepinephrine

KW - Rats

KW - Rats, Sprague-Dawley

KW - Reference Values

KW - Stress, Mechanical

KW - Vasodilation

U2 - 10.1007/s001250050863

DO - 10.1007/s001250050863

M3 - Article

C2 - 9498627

SN - 0012-186X

VL - 41

SP - 34

EP - 39

JO - Diabetologia

JF - Diabetologia

IS - 1

ER -