Fluorescence axial nanotomography with plasmonics

TY - JOUR

T1 - Fluorescence axial nanotomography with plasmonics

AU - Cade, Nic

AU - Fruhwirth, Gilbert

AU - Krasavin, Alexey

AU - Ng, Tony

AU - Richards, David

PY - 2014

Y1 - 2014

N2 - We present a novel imaging technique with super-resolution axial sensitivity, exploiting the changes in fluorescence lifetime above a plasmonic substrate. Using conventional confocal fluorescence lifetime imaging, we show that it is possible to deliver down to 6 nm axial position sensitivity of fluorophores in whole biological cell imaging. We employ this technique to map the topography of the cellular membrane, and demonstrate its application in an investigation of receptor-mediated endocytosis in carcinoma cells.

AB - We present a novel imaging technique with super-resolution axial sensitivity, exploiting the changes in fluorescence lifetime above a plasmonic substrate. Using conventional confocal fluorescence lifetime imaging, we show that it is possible to deliver down to 6 nm axial position sensitivity of fluorophores in whole biological cell imaging. We employ this technique to map the topography of the cellular membrane, and demonstrate its application in an investigation of receptor-mediated endocytosis in carcinoma cells.

U2 - 10.1039/C4FD00198B

DO - 10.1039/C4FD00198B

M3 - Article

SN - 1359-6640

JO - Faraday Discussions

JF - Faraday Discussions

ER -