Fluorogenic substrates for the detection of saliva

James Gooch; Chang Rong Chua; Vincenzo Abbate; Nunzianda Frascione

doi:10.1016/j.fsigss.2017.09.218

Fluorogenic substrates for the detection of saliva

James Gooch, Chang Rong Chua, Vincenzo Abbate, Nunzianda Frascione

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

201 Downloads (Pure)

Abstract

The potential of fluorogenic substrates to detect and identify human saliva in situ was first explored using commercially available substrate Boc-VPR-AMC. The substrate was applied to a range of saliva dilutions deposited on glass microscope slides. A positive fluorescence response was observed immediately after application against each deposit up to a 1:8 dilution. In an attempt to improve assay sensitivity, a novel substrate Ac-VPR-Rho110-Ac, utilising a Rhodamine-110 fluorophore, was prepared using a solid-phase peptide synthesis protocol previously reported by our research group. Application of Ac-VPR-Rho110-Ac to saliva deposits demonstrated a successful increase in assay sensitivity limits, allowing stains of up to 1 in 128 dilution to be detected.

Original language	English
Journal	Forensic Science International: Genetics Supplement Series
Early online date	29 Sept 2017
DOIs	https://doi.org/10.1016/j.fsigss.2017.09.218
Publication status	E-pub ahead of print - 29 Sept 2017

Keywords

Fluorogenic substrates
Saliva detection
Body fluids

Access to Document

10.1016/j.fsigss.2017.09.218

Fluorogenic substrates for the detection_GOOCH_Publishedonline29September2017_GREEN AAM (CC BY-NC-ND)Accepted author manuscript, 281 KBLicence: CC BY-NC-ND

Cite this

@article{b50792bfacfd4720ab7ee435809559a1,

title = "Fluorogenic substrates for the detection of saliva",

abstract = "The potential of fluorogenic substrates to detect and identify human saliva in situ was first explored using commercially available substrate Boc-VPR-AMC. The substrate was applied to a range of saliva dilutions deposited on glass microscope slides. A positive fluorescence response was observed immediately after application against each deposit up to a 1:8 dilution. In an attempt to improve assay sensitivity, a novel substrate Ac-VPR-Rho110-Ac, utilising a Rhodamine-110 fluorophore, was prepared using a solid-phase peptide synthesis protocol previously reported by our research group. Application of Ac-VPR-Rho110-Ac to saliva deposits demonstrated a successful increase in assay sensitivity limits, allowing stains of up to 1 in 128 dilution to be detected.",

keywords = "Fluorogenic substrates, Saliva detection, Body fluids",

author = "James Gooch and Chua, {Chang Rong} and Vincenzo Abbate and Nunzianda Frascione",

year = "2017",

month = sep,

day = "29",

doi = "10.1016/j.fsigss.2017.09.218",

language = "English",

journal = "Forensic Science International: Genetics Supplement Series",

issn = "1875-1768",

publisher = "Elsevier Ireland Ltd",

}

TY - JOUR

T1 - Fluorogenic substrates for the detection of saliva

AU - Gooch, James

AU - Chua, Chang Rong

AU - Abbate, Vincenzo

AU - Frascione, Nunzianda

PY - 2017/9/29

Y1 - 2017/9/29

N2 - The potential of fluorogenic substrates to detect and identify human saliva in situ was first explored using commercially available substrate Boc-VPR-AMC. The substrate was applied to a range of saliva dilutions deposited on glass microscope slides. A positive fluorescence response was observed immediately after application against each deposit up to a 1:8 dilution. In an attempt to improve assay sensitivity, a novel substrate Ac-VPR-Rho110-Ac, utilising a Rhodamine-110 fluorophore, was prepared using a solid-phase peptide synthesis protocol previously reported by our research group. Application of Ac-VPR-Rho110-Ac to saliva deposits demonstrated a successful increase in assay sensitivity limits, allowing stains of up to 1 in 128 dilution to be detected.

AB - The potential of fluorogenic substrates to detect and identify human saliva in situ was first explored using commercially available substrate Boc-VPR-AMC. The substrate was applied to a range of saliva dilutions deposited on glass microscope slides. A positive fluorescence response was observed immediately after application against each deposit up to a 1:8 dilution. In an attempt to improve assay sensitivity, a novel substrate Ac-VPR-Rho110-Ac, utilising a Rhodamine-110 fluorophore, was prepared using a solid-phase peptide synthesis protocol previously reported by our research group. Application of Ac-VPR-Rho110-Ac to saliva deposits demonstrated a successful increase in assay sensitivity limits, allowing stains of up to 1 in 128 dilution to be detected.

KW - Fluorogenic substrates

KW - Saliva detection

KW - Body fluids

U2 - 10.1016/j.fsigss.2017.09.218

DO - 10.1016/j.fsigss.2017.09.218

M3 - Article

SN - 1875-1768

JO - Forensic Science International: Genetics Supplement Series

JF - Forensic Science International: Genetics Supplement Series

ER -

Fluorogenic substrates for the detection of saliva

Abstract

Keywords

Access to Document

Fingerprint

Cite this