Focal electroporation in zebrafish embryos and larvae

TY - JOUR

T1 - Focal electroporation in zebrafish embryos and larvae

AU - Tawk, Marcel

AU - Bianco, Isaac H

AU - Clarke, Jonathan D W

PY - 2009

Y1 - 2009

N2 - A method is described that allows the introduction by electroporation of either small dyes or larger RNA, DNA, or morpholino constructs into single cells or small groups of cells in zebrafish embryos or larvae. The dye or construct is delivered to cells via a patch-like microelectrode that also delivers the electroporation stimulus train. This technique allows the experimenter to target cells of their choice at a particular time of development and at a particular location in the embryo, and is useful for fate mapping, analysing neuronal organisation, ectopic expression and gene knockdown experiments.

AB - A method is described that allows the introduction by electroporation of either small dyes or larger RNA, DNA, or morpholino constructs into single cells or small groups of cells in zebrafish embryos or larvae. The dye or construct is delivered to cells via a patch-like microelectrode that also delivers the electroporation stimulus train. This technique allows the experimenter to target cells of their choice at a particular time of development and at a particular location in the embryo, and is useful for fate mapping, analysing neuronal organisation, ectopic expression and gene knockdown experiments.

KW - Animals

KW - Electroporation

KW - Larva

KW - Animals, Genetically Modified

KW - Fluorescent Dyes

KW - Embryo, Nonmammalian

KW - Zebrafish

KW - Developmental Biology

KW - RNA

KW - Microscopy

KW - DNA

KW - Rhodamines

KW - Time Factors

U2 - 10.1007/978-1-60327-977-2_10

DO - 10.1007/978-1-60327-977-2_10

M3 - Article

C2 - 19378103

SN - 1064-3745

VL - 546

SP - 145

EP - 151

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -