TY - JOUR
T1 - Formulation of vaccines containing CpG oligonucleotides and alum
AU - Aebig, Joan A.
AU - Mullen, Gregory E. D.
AU - Dobrescu, Gelu
AU - Rausch, Kelly
AU - Lambert, Lynn
AU - Ajose-Popoola, Olubunmi
AU - Long, Carole A.
AU - Saul, Allan
AU - Miles, Aaron P.
PY - 2007/6/30
Y1 - 2007/6/30
N2 - CpG oligodeoxynucleotides are potent immunostimulants. For parenterally delivered alum-based vaccines, the immunostimulatory effect of CpG depends on the association of the CpG and antigen to the alum. We describe effects of buffer components on the binding of CPG 7909 to aluminum hydroxide (Alhydrogel), assays for measuring binding of CPG 7909 to alum and CPG 7909 induced dissociation of antigen from the alum. Free CPG 7909 is a potent inducer of IP-10 in mice. However the lack of IP-10 production from formulations containing bound CPG 7909 suggested that CPG 7909 does not rapidly dissociate from the alum after injection. It also suggests that IP-10 assays are not a good basis for potency assays for alum-based vaccines containing CPG 7909. (C) 2007 Elsevier B.V. All rights reserved.
AB - CpG oligodeoxynucleotides are potent immunostimulants. For parenterally delivered alum-based vaccines, the immunostimulatory effect of CpG depends on the association of the CpG and antigen to the alum. We describe effects of buffer components on the binding of CPG 7909 to aluminum hydroxide (Alhydrogel), assays for measuring binding of CPG 7909 to alum and CPG 7909 induced dissociation of antigen from the alum. Free CPG 7909 is a potent inducer of IP-10 in mice. However the lack of IP-10 production from formulations containing bound CPG 7909 suggested that CPG 7909 does not rapidly dissociate from the alum after injection. It also suggests that IP-10 assays are not a good basis for potency assays for alum-based vaccines containing CPG 7909. (C) 2007 Elsevier B.V. All rights reserved.
U2 - 10.1016/j.jim.2007.03.003
DO - 10.1016/j.jim.2007.03.003
M3 - Article
VL - 323
SP - 139
EP - 146
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 2
ER -