Fretibacterium fastidiosum gen. nov., sp. nov., isolated from the human oral cavity

TY - JOUR

T1 - Fretibacterium fastidiosum gen. nov., sp. nov., isolated from the human oral cavity

AU - Vartoukian, Sonia R

AU - Downes, Julia

AU - Palmer, Richard M

AU - Wade, William G

PY - 2012

Y1 - 2012

N2 - SGP1T , a strain belonging to a lineage of the phylum Synergistetes with no previously cultivated representatives was subjected to a comprehensive range of phenotypic and genotypic tests. The strain was dependent for good growth on co-culture with, or extracts from, selected other oral bacteria. Phylogenetic analysis of the full-length 16S rRNA gene showed that the strain represented a novel group within the phylum Synergistetes. A new genus Fretibacterium and the new species Fretibacterium fastidiosum gen. nov. sp. nov. are proposed. The cells are asaccharolytic and major amounts of acetic acid and moderate amounts of propionic acid are produced as end products of metabolism in peptone-yeast extract-glucose broth supplemented with a filtered cell sonicate of Fusobacterium nucleatum (25% v/v). Hydrogen sulphide is produced and gelatin is weakly hydrolysed. The major cellular fatty acids are C14:0, C18:0 and C16:0. The G+C content of the DNA of the type strain is 63 mol%. The type strain of Fretibacterium fastidiosum is SGP1T (=DSM 25557T =JCM 16858T).

AB - SGP1T , a strain belonging to a lineage of the phylum Synergistetes with no previously cultivated representatives was subjected to a comprehensive range of phenotypic and genotypic tests. The strain was dependent for good growth on co-culture with, or extracts from, selected other oral bacteria. Phylogenetic analysis of the full-length 16S rRNA gene showed that the strain represented a novel group within the phylum Synergistetes. A new genus Fretibacterium and the new species Fretibacterium fastidiosum gen. nov. sp. nov. are proposed. The cells are asaccharolytic and major amounts of acetic acid and moderate amounts of propionic acid are produced as end products of metabolism in peptone-yeast extract-glucose broth supplemented with a filtered cell sonicate of Fusobacterium nucleatum (25% v/v). Hydrogen sulphide is produced and gelatin is weakly hydrolysed. The major cellular fatty acids are C14:0, C18:0 and C16:0. The G+C content of the DNA of the type strain is 63 mol%. The type strain of Fretibacterium fastidiosum is SGP1T (=DSM 25557T =JCM 16858T).

U2 - 10.1099/ijs.0.041038-0

DO - 10.1099/ijs.0.041038-0

M3 - Article

C2 - 22493171

SN - 1466-5026

JO - INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY

JF - INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY

ER -