Functional in vivo imaging using fluorescence lifetime light-sheet microscopy

Claire A. Mitchell, Simon P Poland, James Seyforth, Jakub Nedbal, Thomas Gelot, Tahiyat Huq, Gerhard Holst, Robert D. Knight, Simon M. Ameer-Beg*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)
287 Downloads (Pure)

Abstract

Light-sheet microscopy has become an indispensable tool for fast, low phototoxicity volumetric imaging of biological samples, predominantly providing structural or analyte concentration data in its standard format. Fluorescence lifetime imaging microscopy (FLIM) provides functional contrast, but often at limited acquisition speeds and with complex implementation. Therefore, we incorporate a dedicated frequency domain CMOS FLIM camera and intensity-modulated laser into a light-sheet setup to add fluorescence lifetime imaging functionality, allowing the rapid acquisition of volumetric data with concentration independent contrast. We then apply the system to image live transgenic zebrafish, demonstrating the capacity to rapidly collect volumetric FLIM data from an in vivo sample.

Original languageEnglish
Pages (from-to)1269-1272
Number of pages4
JournalOptics Letters
Volume42
Issue number7
DOIs
Publication statusPublished - 23 Mar 2017

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