Functional screenings reveal different requirements for host microRNAs in Salmonella and Shigella infection

Carmen Aguilar, Ana Rita Cruz, Ines Rodrigues Lopes, Claire Maudet, Ushasree Sunkavalli, Ricardo Jorge Silva, Malvika Sharan, Clivia Lisowski, Sara Zaldívar-López, Juan José Garrido, Mauro Giacca, Miguel Mano, Ana Eulalio*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) are increasingly recognized for their role in infection by bacterial pathogens, although the effect of each individual miRNA remains largely unknown. Here, we used a comparative genome-wide microscopy-based functional screening approach to identify miRNAs controlling infection by two bacterial pathogens—Salmonellaenterica serovar Typhimurium and Shigella flexneri. Despite the similarities between these pathogens, we found infections to be controlled by largely non-overlapping subsets of miRNAs, seemingly reflecting different requirements prompted by their distinct intracellular lifestyles. By characterizing a small subset of miRNAs chosen among the strongest inhibitors of Shigella infection, we discovered that miR-3668, miR-4732-5p and miR-6073 exert a selective effect on Shigella infection by impairing bacterial actin-based motility by downregulating N-WASP. Additionally, by identifying let-7i-3p miRNA as a strong inhibitor of Salmonella replication and performing in-depth analysis of its mechanisms of action, we showed that this miRNA specifically inhibits Salmonella infection via modulation of endolysosomal trafficking and the vacuolar environment by targeting the host RGS2 protein. These findings illustrate two paradigms underlying miRNA-mediated regulation of bacterial infection, acting as part of the host response to infection, or as part of bacterial strategies to modulate the host environment and favour pathogenesis.

Original languageEnglish
Pages (from-to)192-205
Number of pages14
JournalNature Microbiology
Volume5
Issue number1
DOIs
Publication statusPublished - 1 Jan 2020

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