Functional specificity of the Xenopus T-domain protein brachyury is conferred by its ability to interact with Smad1

Nigel J Messenger, Christin Kabitschke, Robert Andrews, Donna Grimmer, Ricardo Nunez Miguel, Tom L Blundell, James C Smith, Fiona C Wardle

Research output: Contribution to journalArticlepeer-review

70 Citations (Scopus)

Abstract

Members of the T-box gene family play important and diverse roles in development and disease. Here, we study the functional specificities of the Xenopus T-domain proteins Xbra and VegT, which differ in their abilities to induce gene expression in prospective ectodermal tissue. In particular, VegT induces strong expression of goosecoid whereas Xbra cannot. Our results indicate that Xbra is unable to induce goosecoid because it directly activates expression of Xom, a repressor of goosecoid that acts downstream of BMP signaling. We show that the inability of Xbra to induce goosecoid is imposed by an N-terminal domain that interacts with the C-terminal MH2 domain of Smad1, a component of the BMP signal transduction pathway. Interference with this interaction causes ectopic activation of goosecoid and anteriorization of the embryo. These findings suggest a mechanism by which individual T-domain proteins may interact with different partners to elicit a specific response.
Original languageEnglish
Pages (from-to)599 - 610
Number of pages12
JournalDevelopmental Cell
Volume8
Issue number4
DOIs
Publication statusPublished - Apr 2005

Fingerprint

Dive into the research topics of 'Functional specificity of the Xenopus T-domain protein brachyury is conferred by its ability to interact with Smad1'. Together they form a unique fingerprint.

Cite this