Functionalization of filled radioactive multi-walled carbon nanocapsules by arylation reaction forin vivodelivery of radio-therapy

Agnieszka Gajewska; Julie T.W. Wang; Rebecca Klippstein; Markus Martincic; Elzbieta Pach; Robert Feldman; Jean Claude Saccavini; Gerard Tobias; Belén Ballesteros; Khuloud T. Al-Jamal; Tatiana Da Ros

doi:10.1039/d1tb02195h

Functionalization of filled radioactive multi-walled carbon nanocapsules by arylation reaction forin vivodelivery of radio-therapy

Agnieszka Gajewska, Julie T.W. Wang, Rebecca Klippstein, Markus Martincic, Elzbieta Pach, Robert Feldman, Jean Claude Saccavini, Gerard Tobias, Belén Ballesteros, Khuloud T. Al-Jamal^*, Tatiana Da Ros

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Functionalized multi-walled carbon nanotubes (MWCNTs) containing radioactive salts are proposed as a potential system for radioactivity delivery. MWCNTs are loaded with isotopically enriched 152-samarium chloride (¹⁵²SmCl₃), the ends of the MWCNTs are sealed by high temperature treatment, and the encapsulated¹⁵²Sm is neutron activated to radioactive¹⁵³Sm. The external walls of the radioactive nanocapsules are functionalized through arylation reaction, to introduce hydrophilic chains and increase the water dispersibility of CNTs. The organ biodistribution profiles of the nanocapsules up to 24 h are assessed in naïve mice and different tumor modelsin vivo. By quantitative γ-counting,¹⁵³SmCl₃@MWCNTs-NH₂exhibite high accumulation in organs without leakage of the internal radioactive material to the bloodstream. In the treated mice, highest uptake is detected in the lung followed by the liver and spleen. Presence of tumors in brain or lung does not increase percentage accumulation of¹⁵³SmCl₃@MWCNTs-NH₂in the respective organs, suggesting the absence of the enhanced permeation and retention effect. This study presents a chemical functionalization protocol that is rapid (∼one hour) and can be applied to filled radioactive multi-walled carbon nanocapsules to improve their water dispersibility for systemic administration for their use in targeted radiotherapy.

Original language	English
Pages (from-to)	47-56
Number of pages	10
Journal	Journal of materials chemistry b
Volume	10
Issue number	1
DOIs	https://doi.org/10.1039/d1tb02195h
Publication status	Published - 7 Jan 2022

Access to Document

10.1039/d1tb02195h

Cite this

Gajewska, A., Wang, J. T. W., Klippstein, R., Martincic, M., Pach, E., Feldman, R., Saccavini, J. C., Tobias, G., Ballesteros, B., Al-Jamal, K. T., & Da Ros, T. (2022). Functionalization of filled radioactive multi-walled carbon nanocapsules by arylation reaction forin vivodelivery of radio-therapy. Journal of materials chemistry b, 10(1), 47-56. https://doi.org/10.1039/d1tb02195h

@article{acfdc86856fd471ba95567390fdedb1e,

title = "Functionalization of filled radioactive multi-walled carbon nanocapsules by arylation reaction forin vivodelivery of radio-therapy",

abstract = "Functionalized multi-walled carbon nanotubes (MWCNTs) containing radioactive salts are proposed as a potential system for radioactivity delivery. MWCNTs are loaded with isotopically enriched 152-samarium chloride (152SmCl3), the ends of the MWCNTs are sealed by high temperature treatment, and the encapsulated152Sm is neutron activated to radioactive153Sm. The external walls of the radioactive nanocapsules are functionalized through arylation reaction, to introduce hydrophilic chains and increase the water dispersibility of CNTs. The organ biodistribution profiles of the nanocapsules up to 24 h are assessed in na{\"i}ve mice and different tumor modelsin vivo. By quantitative γ-counting,153SmCl3@MWCNTs-NH2exhibite high accumulation in organs without leakage of the internal radioactive material to the bloodstream. In the treated mice, highest uptake is detected in the lung followed by the liver and spleen. Presence of tumors in brain or lung does not increase percentage accumulation of153SmCl3@MWCNTs-NH2in the respective organs, suggesting the absence of the enhanced permeation and retention effect. This study presents a chemical functionalization protocol that is rapid (∼one hour) and can be applied to filled radioactive multi-walled carbon nanocapsules to improve their water dispersibility for systemic administration for their use in targeted radiotherapy.",

author = "Agnieszka Gajewska and Wang, {Julie T.W.} and Rebecca Klippstein and Markus Martincic and Elzbieta Pach and Robert Feldman and Saccavini, {Jean Claude} and Gerard Tobias and Bel{\'e}n Ballesteros and Al-Jamal, {Khuloud T.} and {Da Ros}, Tatiana",

note = "Funding Information: We thank Thomas Swan & Co. Ltd for supplying the Eli-carb? MWCNTs. This work was supported by European Union's Seventh Framework Programme FP7, Project ?RADDEL? [grant number 290023], Worldwide Cancer Research [grant number 12-1054], Biotechnology and Biological Sciences Research Council [grant number BB/J008656/1], European Union HORIZON 2020 MSCA RISE 2016, Project Carbo-Immap [grant number 734381], ?Severo Ochoa? Programme for Centres of Excellence in R&D [grant numbers SEV-2015-0496, SEV-2017-0706], and Generalitat de Catalunya 2017 [grant number SGR 327]. Funding Information: We thank Thomas Swan & Co. Ltd for supplying the Eli-carbs MWCNTs. This work was supported by European Union{\textquoteright}s Seventh Framework Programme FP7, Project {\textquoteleft}{\textquoteleft}RADDEL{\textquoteright}{\textquoteright} [grant number 290023], Worldwide Cancer Research [grant number 12-1054], Biotechnology and Biological Sciences Research Council [grant number BB/J008656/1], European Union HORIZON 2020 MSCA RISE 2016, Project Carbo-Immap [grant number 734381], {\textquoteleft}{\textquoteleft}Severo Ochoa{\textquoteright}{\textquoteright} Programme for Centres of Excellence in R&D [grant numbers SEV-2015-0496, SEV-2017-0706], and Generalitat de Catalunya 2017 [grant number SGR 327]. Publisher Copyright: {\textcopyright} The Royal Society of Chemistry 2021.",

year = "2022",

month = jan,

day = "7",

doi = "10.1039/d1tb02195h",

language = "English",

volume = "10",

pages = "47--56",

journal = "Journal of materials chemistry b",

issn = "2050-750X",

publisher = "Royal Society of Chemistry",

number = "1",

}

Gajewska, A, Wang, JTW, Klippstein, R, Martincic, M, Pach, E, Feldman, R, Saccavini, JC, Tobias, G, Ballesteros, B, Al-Jamal, KT & Da Ros, T 2022, 'Functionalization of filled radioactive multi-walled carbon nanocapsules by arylation reaction forin vivodelivery of radio-therapy', Journal of materials chemistry b, vol. 10, no. 1, pp. 47-56. https://doi.org/10.1039/d1tb02195h

TY - JOUR

T1 - Functionalization of filled radioactive multi-walled carbon nanocapsules by arylation reaction forin vivodelivery of radio-therapy

AU - Gajewska, Agnieszka

AU - Wang, Julie T.W.

AU - Klippstein, Rebecca

AU - Martincic, Markus

AU - Pach, Elzbieta

AU - Feldman, Robert

AU - Saccavini, Jean Claude

AU - Tobias, Gerard

AU - Ballesteros, Belén

AU - Al-Jamal, Khuloud T.

AU - Da Ros, Tatiana

N1 - Funding Information: We thank Thomas Swan & Co. Ltd for supplying the Eli-carb? MWCNTs. This work was supported by European Union's Seventh Framework Programme FP7, Project ?RADDEL? [grant number 290023], Worldwide Cancer Research [grant number 12-1054], Biotechnology and Biological Sciences Research Council [grant number BB/J008656/1], European Union HORIZON 2020 MSCA RISE 2016, Project Carbo-Immap [grant number 734381], ?Severo Ochoa? Programme for Centres of Excellence in R&D [grant numbers SEV-2015-0496, SEV-2017-0706], and Generalitat de Catalunya 2017 [grant number SGR 327]. Funding Information: We thank Thomas Swan & Co. Ltd for supplying the Eli-carbs MWCNTs. This work was supported by European Union’s Seventh Framework Programme FP7, Project ‘‘RADDEL’’ [grant number 290023], Worldwide Cancer Research [grant number 12-1054], Biotechnology and Biological Sciences Research Council [grant number BB/J008656/1], European Union HORIZON 2020 MSCA RISE 2016, Project Carbo-Immap [grant number 734381], ‘‘Severo Ochoa’’ Programme for Centres of Excellence in R&D [grant numbers SEV-2015-0496, SEV-2017-0706], and Generalitat de Catalunya 2017 [grant number SGR 327]. Publisher Copyright: © The Royal Society of Chemistry 2021.

PY - 2022/1/7

Y1 - 2022/1/7

N2 - Functionalized multi-walled carbon nanotubes (MWCNTs) containing radioactive salts are proposed as a potential system for radioactivity delivery. MWCNTs are loaded with isotopically enriched 152-samarium chloride (152SmCl3), the ends of the MWCNTs are sealed by high temperature treatment, and the encapsulated152Sm is neutron activated to radioactive153Sm. The external walls of the radioactive nanocapsules are functionalized through arylation reaction, to introduce hydrophilic chains and increase the water dispersibility of CNTs. The organ biodistribution profiles of the nanocapsules up to 24 h are assessed in naïve mice and different tumor modelsin vivo. By quantitative γ-counting,153SmCl3@MWCNTs-NH2exhibite high accumulation in organs without leakage of the internal radioactive material to the bloodstream. In the treated mice, highest uptake is detected in the lung followed by the liver and spleen. Presence of tumors in brain or lung does not increase percentage accumulation of153SmCl3@MWCNTs-NH2in the respective organs, suggesting the absence of the enhanced permeation and retention effect. This study presents a chemical functionalization protocol that is rapid (∼one hour) and can be applied to filled radioactive multi-walled carbon nanocapsules to improve their water dispersibility for systemic administration for their use in targeted radiotherapy.

AB - Functionalized multi-walled carbon nanotubes (MWCNTs) containing radioactive salts are proposed as a potential system for radioactivity delivery. MWCNTs are loaded with isotopically enriched 152-samarium chloride (152SmCl3), the ends of the MWCNTs are sealed by high temperature treatment, and the encapsulated152Sm is neutron activated to radioactive153Sm. The external walls of the radioactive nanocapsules are functionalized through arylation reaction, to introduce hydrophilic chains and increase the water dispersibility of CNTs. The organ biodistribution profiles of the nanocapsules up to 24 h are assessed in naïve mice and different tumor modelsin vivo. By quantitative γ-counting,153SmCl3@MWCNTs-NH2exhibite high accumulation in organs without leakage of the internal radioactive material to the bloodstream. In the treated mice, highest uptake is detected in the lung followed by the liver and spleen. Presence of tumors in brain or lung does not increase percentage accumulation of153SmCl3@MWCNTs-NH2in the respective organs, suggesting the absence of the enhanced permeation and retention effect. This study presents a chemical functionalization protocol that is rapid (∼one hour) and can be applied to filled radioactive multi-walled carbon nanocapsules to improve their water dispersibility for systemic administration for their use in targeted radiotherapy.

UR - http://www.scopus.com/inward/record.url?scp=85121898074&partnerID=8YFLogxK

U2 - 10.1039/d1tb02195h

DO - 10.1039/d1tb02195h

M3 - Article

AN - SCOPUS:85121898074

SN - 2050-750X

VL - 10

SP - 47

EP - 56

JO - Journal of materials chemistry b

JF - Journal of materials chemistry b

IS - 1

ER -

Functionalization of filled radioactive multi-walled carbon nanocapsules by arylation reaction forin vivodelivery of radio-therapy

Abstract

Access to Document

Other files and links

Fingerprint

Cite this