Further characterisation of a thromboembolic model of stroke in the rat

TY - JOUR

T1 - Further characterisation of a thromboembolic model of stroke in the rat

AU - Beech, J S

AU - Williams, S C R

AU - Campbell, C A

AU - Bath, P M W

AU - Parsons, A A

AU - Hunter, A J

AU - Menon, D K

PY - 2001/3/23

Y1 - 2001/3/23

N2 - We have used magnetic resonance imaging (MRI) techniques to characterise a rat model of thromboembolic stroke. The consequences of acute perfusion deficit associated with a middle cerebral artery occlusion (MCAo) by a newly formed thrombus was mapped by interrogation of the tissue oxygenation status using gradient echo methods and production of T2* maps. Final infarct size was subsequently assessed at 24-h post-ischaemia by histology with 2,3,5-triphenyltetrazolium chloride (TTC) staining. Animals displayed an infarct volume of 178.7 +/- 84.2 mm(3) (mean +/-S.D.) with a large coefficient of variation (47%) and range of values (85.6-265.5 mm(3)). This variability provided us with an opportunity to assess the relationships between early imaging observations and eventual infarct size. For a single cerebral slice, at the centre of the MCA territory, a relationship between the area of reduced T2* at 1 and 2 h post MCAo correlated highly with final lesion area (Spearman rank correlation, r=0.98. P84.2 vs. 243.3 +/- 50.1 mm(3), mean +/-S.D., Student's t-test P=0.046) and showed a greater variability (coefficient of variations: 47% vs. 21%). These data underline the relative variability of this embolic model and provide important preliminary information regarding the value of early changes in T2* in predicting eventual infarct size. (C) 2001 Elsevier Science B.V. All rights reserved.

AB - We have used magnetic resonance imaging (MRI) techniques to characterise a rat model of thromboembolic stroke. The consequences of acute perfusion deficit associated with a middle cerebral artery occlusion (MCAo) by a newly formed thrombus was mapped by interrogation of the tissue oxygenation status using gradient echo methods and production of T2* maps. Final infarct size was subsequently assessed at 24-h post-ischaemia by histology with 2,3,5-triphenyltetrazolium chloride (TTC) staining. Animals displayed an infarct volume of 178.7 +/- 84.2 mm(3) (mean +/-S.D.) with a large coefficient of variation (47%) and range of values (85.6-265.5 mm(3)). This variability provided us with an opportunity to assess the relationships between early imaging observations and eventual infarct size. For a single cerebral slice, at the centre of the MCA territory, a relationship between the area of reduced T2* at 1 and 2 h post MCAo correlated highly with final lesion area (Spearman rank correlation, r=0.98. P84.2 vs. 243.3 +/- 50.1 mm(3), mean +/-S.D., Student's t-test P=0.046) and showed a greater variability (coefficient of variations: 47% vs. 21%). These data underline the relative variability of this embolic model and provide important preliminary information regarding the value of early changes in T2* in predicting eventual infarct size. (C) 2001 Elsevier Science B.V. All rights reserved.

UR - http://www.scopus.com/inward/record.url?scp=0035937528&partnerID=8YFLogxK

U2 - 10.1016/S0006-8993(00)03331-X

DO - 10.1016/S0006-8993(00)03331-X

M3 - Article

SN - 1873-2747

VL - 895

SP - 18

EP - 24

JO - Brain Research

JF - Brain Research

IS - 1-2

ER -