FUS-SMN Protein Interactions Link the Motor Neuron Diseases ALS and SMA

Tomohiro Yamazaki, Shi Chen, Yong Yu, Biao Yan, Tyler C. Haertlein, Monica A. Carrasco, Juan C. Tapia, Bo Zhai, Rita Das, Melanie Lalancette-Hebert, Aarti Sharma, Siddharthan Chandran, Gareth Sullivan, Agnes Lumi Nishimura, Christopher E. Shaw, Steve P. Gygi, Neil A. Shneider, Tom Maniatis, Robin Reed*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

203 Citations (Scopus)

Abstract

Mutations in the RNA binding protein FUS cause amyotrophic lateral sclerosis (ALS), a fatal adult motor neuron disease. Decreased expression of SMN causes the fatal childhood motor neuron disorder spinal muscular atrophy (SMA). The SMN complex localizes in both the cytoplasm and nuclear Gems, and loss of Gems is a cellular hallmark of fibroblasts in patients with SMA. Here, we report that FUS associates with the SMN complex, mediated by U1 snRNP and by direct interactions between FUS and SMN. Functionally, we show that FUS is required for Gem formation in HeLa cells, and expression of FUS containing a severe ALS-causing mutation (R495X) also results in Gem loss. Strikingly, a reduction in Gems is observed in ALS patient fibroblasts expressing either mutant FUS or TDP-43, another ALS-causing protein that interacts with FUS. The physical and functional interactions among SMN, FUS, TDP-43, and Gems indicate that ALS and SMA share a biochemical pathway, providing strong support for the view that these motor neuron diseases are related.

Original languageEnglish
Pages (from-to)799-806
Number of pages8
JournalCell Reports
Volume2
Issue number4
DOIs
Publication statusPublished - Oct 2012

Keywords

  • TDP-43
  • MESSENGER-RNA
  • COMPLEX
  • FUS/TLS
  • SPINAL MUSCULAR-ATROPHY
  • AMYOTROPHIC-LATERAL-SCLEROSIS
  • IDENTIFICATION
  • SURVIVAL
  • HEXANUCLEOTIDE REPEAT
  • MUTATIONS

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