G protein βγ subunits inhibit TRPM3 ion channels in sensory neurons

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Abstract

Transient receptor potential (TRP) ion channels in peripheral sensory neurons are functionally regulated by phosphoinositide PI(4,5)P2 hydrolysis and changes in the level of protein kinase mediated phosphorylation following activation of various G protein coupled receptors. We now show that the activity of TRPM3 expressed in mouse dorsal root ganglion (DRG) neurons is inhibited by agonists of the Gi-coupled µ opioid, GABA-B and NPY receptors. These agonist effects are mediated by direct inhibition of TRPM3 by Gβγ subunits, rather than by a canonical cAMP mediated mechanism. The activity of TRPM3 in DRG neurons is also negatively modulated by tonic, constitutive GPCR activity as TRPM3 responses can be potentiated by GPCR inverse agonists. GPCR regulation of TRPM3 is also seen in vivo where Gi/o GPCRs agonists inhibited and inverse agonists potentiated TRPM3 mediated nociceptive behavioural responses.

Original languageEnglish
Article numbere26138
Number of pages22
JournaleLife
Volume6
Early online date15 Aug 2017
DOIs
Publication statusPublished - 15 Aug 2017

Keywords

  • Journal Article

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