Research output: Contribution to journal › Article › peer-review
R. Alhabbab, P. Blair, L. A. Smyth, K. Ratnasothy, Q. Peng, A. Moreau, R. Lechler, R. Elgueta, G. Lombardi
Original language | English |
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Article number | 2725 |
Journal | Scientific Reports |
Volume | 8 |
Early online date | 9 Feb 2018 |
DOIs | |
Accepted/In press | 4 Jan 2018 |
E-pub ahead of print | 9 Feb 2018 |
Published | 2018 |
Additional links |
Galectin-1 is required_ALHABBAB_Accepted4Jan2018_GOLD VoR CC BY
s41598_018_19965_z.pdf, 1.17 MB, application/pdf
Uploaded date:15 Apr 2020
Version:Final published version
Licence:CC BY
Galectin-1 (Gal-1) is required for the development of B cells in the bone marrow (BM), however very little is known about the contribution of Gal-1 to the development of B cell regulatory function. Here, we report an important role for Gal-1 in the induction of B cells regulatory function. Mice deficient of Gal-1 (Gal-1-/-) showed significant loss of Transitional-2 (T2) B cells, previously reported to include IL-10+ regulatory B cells. Gal-1-/- B cells stimulated in vitro via CD40 molecules have impaired IL-10 and Tim-1 expression, the latter reported to be required for IL-10 production in regulatory B cells, and increased TNF-α expression compared to wild type (WT) B cells. Unlike their WT counterparts, T2 and T1 Gal-1-/- B cells did not suppress TNF-α expression by CD4+ T cells activated in vitro with allogenic DCs (allo-DCs), nor were they suppressive in vivo, being unable to delay MHC-class I mismatched skin allograft rejection following adoptive transfer. Moreover, T cells stimulated with allo-DCs show an increase in their survival when co-cultured with Gal-1-/- T2 and MZ B cells compared to WT T2 and MZ B cells. Collectively, these data suggest that Gal-1 contributes to the induction of B cells regulatory function.
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