Abstract
By using a microarray screen to compare gene responses after sterile laser wounding of wild-type and 'macrophageless' serpent mutant Drosophila embryos, we show the wound-induced programmes that are independent of a pathogenic response and distinguish which of the genes are macrophage dependent. The evolutionarily conserved nature of this response is highlighted by our finding that one such new inflammation-associated gene, growth arrest and DNA damage-inducible gene 45 (GADD45), is upregulated in both Drosophila and murine repair models. Comparison of unwounded wild-type and serpent mutant embryos also shows a portfolio of 'macrophage-specific' genes, which suggest analogous functions with vertebrate inflammatory cells. Besides identifying the various classes of wound- and macrophage-related genes, our data indicate that sterile injury per se, in the absence of pathogens, triggers induction of a 'pathogen response', which might prime the organism for what is likely to be an increased risk of infection.
| Original language | English |
|---|---|
| Pages (from-to) | 465-71 |
| Number of pages | 7 |
| Journal | EMBO Reports |
| Volume | 9 |
| Issue number | 5 |
| Early online date | 23 Mar 2008 |
| DOIs | |
| Publication status | Published - May 2008 |
Keywords
- Animals
- Drosophila
- Drosophila Proteins
- Embryo, Nonmammalian
- GATA Transcription Factors
- Gene Expression
- Green Fluorescent Proteins
- Hemocytes
- Homozygote
- Intracellular Signaling Peptides and Proteins
- Macrophages
- Mutation
- Oligonucleotide Array Sequence Analysis
- Up-Regulation
- Wounds and Injuries