Gene induction following wounding of wild-type versus macrophage-deficient Drosophila embryos

Brian Stramer, Mark Winfield, Tanya Shaw, Thomas H Millard, Sarah Woolner, Paul Martin

Research output: Contribution to journalArticlepeer-review

46 Citations (Scopus)

Abstract

By using a microarray screen to compare gene responses after sterile laser wounding of wild-type and 'macrophageless' serpent mutant Drosophila embryos, we show the wound-induced programmes that are independent of a pathogenic response and distinguish which of the genes are macrophage dependent. The evolutionarily conserved nature of this response is highlighted by our finding that one such new inflammation-associated gene, growth arrest and DNA damage-inducible gene 45 (GADD45), is upregulated in both Drosophila and murine repair models. Comparison of unwounded wild-type and serpent mutant embryos also shows a portfolio of 'macrophage-specific' genes, which suggest analogous functions with vertebrate inflammatory cells. Besides identifying the various classes of wound- and macrophage-related genes, our data indicate that sterile injury per se, in the absence of pathogens, triggers induction of a 'pathogen response', which might prime the organism for what is likely to be an increased risk of infection.

Original languageEnglish
Pages (from-to)465-71
Number of pages7
JournalEMBO Reports
Volume9
Issue number5
Early online date23 Mar 2008
DOIs
Publication statusPublished - May 2008

Keywords

  • Animals
  • Drosophila
  • Drosophila Proteins
  • Embryo, Nonmammalian
  • GATA Transcription Factors
  • Gene Expression
  • Green Fluorescent Proteins
  • Hemocytes
  • Homozygote
  • Intracellular Signaling Peptides and Proteins
  • Macrophages
  • Mutation
  • Oligonucleotide Array Sequence Analysis
  • Up-Regulation
  • Wounds and Injuries

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