Generation and application of TGFβ-educated human Vγ9Vδ2 T cells

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Clinical trials that tested the antitumor activity of γδ T cells have been mostly unsuccessful. To address this, we expanded human Vγ9Vδ2 T cells in TGFβ1, a cytokine which enhances their viability, trafficking properties, and intrinsic antitumor activity. This protocol summarizes the production and in vitro functional characterization of TGFβ1 educated human Vγ9Vδ2 cells and highlights their compatibility with chimeric antigen receptor (CAR) engineering. We also describe in vivo testing of the antitumor activity of these CAR T cells in mice. For complete details on the use and execution of this protocol, please refer to Beatson et al. (2021).

Original languageEnglish
Article number101319
Pages (from-to)101319
Number of pages22
JournalSTAR Protocols
Issue number2
Early online date22 Apr 2022
Publication statusPublished - 17 Jun 2022


  • Biotechnology and bioengineering
  • Cancer
  • Cell Biology
  • Cell culture
  • Cell-based Assays
  • Flow Cytometry/Mass Cytometry
  • Health Sciences
  • Immunology
  • Model Organisms
  • Molecular Biology


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